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https://www.selleckchem.com/products/c381.html The antioxidant potential and antiproliferative activity of the extracts of seeds (Black Cummins) and honey formulations are to be explored. The gas chromatography-mass spectrum (GC-MS) and Thin Layer Chromatography (TLC) fingerprint o and Honey formulation revealed alkaloid, saponin, volatile oil, flavonoid, glycosides, sugar, and phenolic compound in the extract. GC-MS profiling of the cold extract of seeds and honey formulation shows peaks for eleven fractions of compounds. Using TLC, the phenolic compounds of and honey formulations were separated. The current study discovers the cytotoxic effect of black Cummins seeds and honey formulation on human ovarian cancer (PA-1) cell line as assessed by MTT assay. PA-1 cells were inhibited with the increasing concentration of seeds extract and honey formulation. The study validates the importance of the tested extracts in the treatment of cancer. The study validates the importance of the tested extracts in the treatment of cancer. Tacrolimus is a powerful immunosuppressant and has been widely used in organ transplantation. In order to further explore the role of tacrolimus in liver transplantation, we conducted network pharmacology analysis. GSE100155 was obtained from the GEO database, and the DEGs of liver transplantation were analyzed. The 2D structure of tacrolimus was obtained from the National Library of Medicine, and the pharmacophore model of tacrolimus was predicted using the online tool pharmmapper. Then a network of tacrolimus and target genes was constructed through network pharmacology, and visualization and GO enrichment analysis was performed through Cytoscape. In addition, we also analyzed the correlation between key genes and immune infiltrating cells. The data of GSE84908 was used to verify the changes of key gene expression levels after tacrolimus treatment. The results of network pharmacological analysis showed that tacrolimus had 43 target genes, and the

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