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https://www.selleckchem.com/products/vtx-27.html PURPOSE To introduce a novel in vivo method (Visual Acuity Dry Up; VADU) for testing the visual performance during the dewetting of the pre-lens tear film on a contact lens (CL). METHODS Thirty-nine subjects were fitted with daily disposable CL (Nelfilcon A). Visual performance and dewetting characteristics of the pre-lens tear film were simultaneously assessed using a modified multifunctional topographer (Keratograph 5 M, OCULUS Optikgeräte GmbH, Wetzlar, GERMANY) and the Non-Invasive Keratograph Dry-Up Time (NIKDUT) method. Measures were taken after five minutes and eight hours of CL wear and included the Visual Acuity Dry-Up Time (VADUT; time between last blink and visual breakdown) and the Visual Acuity Dry-Up Area (VADUA; dewetted CL area at visual breakdown) at one (VA+0.1logMAR), two (VA+0.2logMAR) and three (VA+0.3logMAR) log units below maximal visual acuity (VA). RESULTS Participants lost one, two or three log units of VA after 13.1 ± 17.4 s, 20.83 ± 21.81, 34.67 ± 29.11 (VADUT), corresponding to a dewetted CL area of 4.82 ± 6.64 mm², 9.5 ± 8.26 and 13.0 ± 8.68 (VADUA), respectively. Differences in VADUT und VADUA for all visual requirements were significant (all p 0.05). A median VA loss of one log unit occurred five seconds after the first dewetting. CONCLUSIONS The novel VADU method can be used to analyze the role of the tear film stability on the visual performance during CL wear. Hereby, visual loss is quantified based on the threshold definition of the psychometric function. BACKGROUND Few effective treatments are available for male pattern hair loss (MPHL) and especially for female pattern hair loss (FPHL). Recently, cell-based therapies using autologous or allogeneic cells have been used clinically. OBJECTIVE We examined the safety and efficacy of autologous cell-based therapy using dermal sheath cup (DSC) cells to treat MPHL and FPHL. METHODS DSCs dissected from occipital hair follicles were culture
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