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The relationship between fouling development in a continuous laboratory-scale membrane reactor (MBR/Lab) and the membrane material was investigated using flat-sheet membranes prepared from four materials (polyvinylidene difluoride (PVDF), polyethersulfone, chlorinated polyvinyl chloride, and polytetrafluoroethylene). Further, the characteristics of the suspension liquid in MBR/Lab were compared with those of samples from actual wastewater treatment plants. It was found that, in addition to the membrane material's own characteristics, the structural vulnerability of the membranes had a determining effect on fouling development. The PVDF membrane showed the highest transmembrane pressure during MBR operation and its surface experienced significant damage because of the shearing stress caused by aeration, resulting in the penetration of the membrane by the fouling compounds. The characteristics of suspension liquid in MBR/Lab were almost similar to those in the MBR at a night-soil treatment plant and the aeration tank of a sewage treatment plant. In this study, a lab-scale multiple draft tubes airlift loop membrane bioreactor (Mt-ALMBR) was used for treating acidic 7-amino cephalosporanic acid (7-ACA) wastewater under different pHs (3.54-6.20) and hydraulic retention time (HRT) (48 h, 36 h, 24 h and 16 h). https://www.selleckchem.com/products/peficitinb-asp015k-jnj-54781532.html During about 200 days operation, under HRT of 48 h and pH condition about 6.0, the optimum average COD and BOD5 removal rates were reach to 84.4 ± 2.1% and 94.9 ± 0.8%, and the highest 7-ACA removal rate also observed as 77.6%. Biodegradation, membrane rejection, hydrolysis and sludge adsorption were the four main pathways of 7-ACA removal. With the increase of pH, biodegradation, membrane rejection and hydrolysis had significant positive impacts on 7-ACA removal, while adsorption had a negative impact. Moreover, mathematical models for 7-ACA removal rate and pH were calculated to guide the operation of Mt-ALMBR. Biodegradation was the main pathway to remove 7-ACA when pH was >4.17. In this work, four batch tests were conducted to comprehensively explore the effects of free nitrous acid (FNA) accumulation on nitrous oxide (N2O) emissions in a novel energy-saving and N2O-reducing static/oxic/anoxic (SOA) process. With the accumulation of FNA, the N2O emission factor increased from 1.51% to 4.32%, and the N2O emission ratio contributed by ammonia-oxidizing bacteria (AOB) increased from 74.0% to 78.6%, accordingly. Mechanism studies show that produced FNA and weakened aerobic metabolism bring synergy to competition between reductases. Aeration conditions and FNA cytotoxicity exert a greater impact on nitrite-oxidizing bacteria than on AOB, thus enhancing the potential for nitrite accumulation. Considering the removal of nitrogen and phosphorus and the reduction of N2O emissions in the SOA process, it is feasible to keep the average dissolved oxygen above 2.0 mg/L under the premise of nitrite accumulation. Giant reed was the first time used for photo-fermentative hydrogen production with HAU-M1 bacteria. Effects of NaOH and Ca(OH)2 pretreatments of giant reed on structural changes, enzymatic digestibility, hydrogen production, and energy conversion efficiency were evaluated. Compared to Ca(OH)2 pretreatment, NaOH pretreatment removed more dry matter and lignin at the same loading. The highest glucose yield (44.9%) of NaOH pretreatment was 1.74-fold higher than that of Ca(OH)2 pretreatment. 20% NaOH pretreated giant reed biomass achieved the highest hydrogen yield (98.3 mL/g TS), which was 20% and 70% higher than the highest level of Ca(OH)2 pretreated (20% Ca(OH)2) and untreated giant reed, respectively. Only giant reed biomass pretreated with 20% NaOH resulted in a significant (p less then 0.05) increase (25%) in energy conversion efficiency. An increasing interest is devoted to combined microbial electrolysis cell-anaerobic digestion (MEC-AD) system which could convert waste activated sludge into biogas. In this study series tests were initially conducted to study the effect of alkaline pretreatment on AD system and the results showed that alkaline pretreatment could promote the dissolution of organic matters in the sludge and thus improve the methane production. Then, the methane production in combined MEC-AD system fed with alkaline-pretreated sludge was investigated. The results indicated that the methane productions increased by 37% and 42% when applied voltage was 0.5 V and 0.8 V. The microbial electrochemical system strongly promoted the growth of Euryarchaeota (Methanosaeta and Methanobacterium). Meanwhile, the abundance of Paraclostridium increased from 17.9% to 38.5% when applied voltage was 0.8 V, suggesting an enhanced fermentation and acetogenesis process. The results of energy balance estimation indicated that MEC-AD system at 0.5 V could achieve higher net energy output. Anaerobic high-solid treatment (HST) for processing food waste and biogas production is a viable technology with considerable commercial potential. In this study, we examined and compared mesophilic and thermophilic industrial-scale plug-flow digesters. The HSTs demonstrated reasonable biogas yields from food waste (0.4-0.6 Nm3 CH4/kg volatile solids). However, during operation at thermophilic conditions ammonia inhibition (~2 g NH3-N/L) and acid accumulation (6-14 g/L) caused severe process disturbance. Microbial community structures diverged between the processes, with temperature appearing to be a strong driver. A unique feature of the thermophilic HSTs was high abundance of the uncultivated Clostridia group MBA03 and temperature fluctuations in one mesophilic HST were linked to drastically decreased abundance of methanogens and relative abundance of Cloacimonetes. The process data obtained in this study clearly demonstrate both potential and challenges in HST of food waste but also possibilities for management approaches to tackle process imbalance and restore process function. Nitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R-SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC.
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