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https://www.selleckchem.com/products/rk-701.html Aim Cooled radiofrequency ablation (CRFA) has demonstrated efficacy in the management of knee pain caused by osteoarthritis. A typical procedure involves fluoroscopic placement of internally cooled radiofrequency probes to ensure the probes are located near target genicular nerves. Patients & methods A new technique was developed to perform CRFA using ultrasound (US) guidance. Patient outcomes were reported using a telephone survey. Results Ablation procedures were successfully performed using US guidance. A total of 51 patients were retrospectively identified for telephone screening. A total of 22 patients completed the telephone questionnaire. There were no safety concerns identified and the majority of patients reported satisfactory outcomes. Conclusion Patient outcomes demonstrate that US-guided CRFA procedures provide pain relief and functional improvement. Further studies are needed to compare the efficacy of US-guided CRFA to fluoroscopy-guided CRFA procedures.The inosine triphosphate pyrophosphatase (ITPA) protein is responsible for removing noncanonical purine nucleoside triphosphates from intracellular nucleotide pools. Absence of ITPA results in genomic instability and increased levels of inosine in DNA and RNA. The proline to threonine substitution at position 32 (P32T) affects roughly 15% of the global population and can modulate treatment outcomes for cancer, lupus, and hepatitis C patients. The substitution of arginine with cysteine at position 178 (R178C) is extremely uncommon and has only been reported in a small cohort of early infantile encephalopathy patients suggesting that a functional ITPA protein is required for life in humans. Here we present molecular dynamic simulations that describe the structure and dynamics of the wild-type ITPA homodimer and two of its clinically relevant mutants, P32T and R178C. The simulation results indicate that both the P32T and R178C mutations alter the structure a
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