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The fibrinolytic activity in Korean traditional fermented food, Jotgal (pickled fish) was identified. Though the fibrinolytic activity could vary in different kinds of Jotgal, this activity seems to be produced by microorganisms during the natural fermentation stage. From Gonjaengijot (pickled opossum shrimp), two novel fibrinolytic enzymes named by JP-I and JP-II, have been purified by ethanol precipitation, Bio-GEL P-100 gel filtration, and DEAE-cellulose ion-exchange chromatography. Compared to the crude enzyme extract, the specific activity of the JP-I and JP-II increased 258, 85-fold with the recovery of 22.1, 8.5%, respectively. The molecular weights of both enzymes were estimated as 36 kDa on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimal condition for fibrinolytic activity of JP-I was at 50°C and pH 8.1, while that of JP-II was at 45°C and 9.9. Both enzymes were stable at a broad range of pH (5.0 to 10.5) and have metalloprotease nature. From these results, it conclhrombolytic agent.Next-generation devices for low-latency and seamless communication are envisioned to revolutionize information processing, which would directly impact human lives, technologies, and societies. The ever-increasing demand for wireless data traffic can be fulfilled by the terahertz band, which has received tremendous attention as the final frontier of the radio spectrum. However, attenuation due to atmospheric humidity and free-space path loss significantly limits terahertz signal propagation. High-gain antennas with directional radiation and reconfigurable beam steering are indispensable for loss compensation and terahertz signal processing, which are associated with spatial and temporal dimensions, respectively. Here, experimental demonstration of a spatiotemporal dielectric metasurface for unidirectional propagation and ultrafast spatial beam steering of terahertz waves is shown. The spatial dimension of the metasurface provides a solution to eliminate backscattering of collimated unidirectional propagation of the terahertz wave with steerable directionality. Temporal modulation of the spatial optical properties enables ultrafast reconfigurable beam steering. https://www.selleckchem.com/products/gdc-0068.html Silicon-based spatiotemporal devices amalgamate the rich physics of metasurfaces and technologies that are promising for overcoming the bottlenecks of future terahertz communication, such as high-speed and secure wireless data transmission, beamforming and ultrafast data processing.19 F magnetic resonance imaging (MRI) is an promising emerging imaging modality. Specifically, the inherent sensitivity of the spin-lattice relaxation time ( T 1 ) of 19 F nuclei to oxygen partial pressure makes 19 F MRI an attractive non-invasive approach to quantify tissue oxygenation in a spatiotemporal manner. There remain, however, few materials with the adequate sensitivity to be used as oxygen-sensitive 19 F MRI agents. Herein, we report highly fluorinated monomers that provide a platform to realize water-soluble, partially fluorinated copolymers as 19 F MRI agents with the required sensitivity to quantify solution oxygenation at clinically relevant magnetic field strengths. The synthesis of a systematic library of partially fluorinated copolymers enabled a comprehensive evaluation of copolymer structure-property relationships relevant to 19 F MRI. The highest-performing material composition demonstrated a signal-to-noise ratio that corresponded to an apparent 19 F density of 220 mM, which surpasses the threshold of 126 mM 19 F required for visualization on a three Tesla clinical MRI. Furthermore, the T 1 of these high performing materials demonstrated a linear relationship with solution oxygenation, with oxygen sensitivity reaching 240×10 -5 mmHg -1 s -1 . The relationships between material composition and 19 F MRI performance identified herein suggest general structure-property criteria for the further improvement of modular, water-soluble 19 F MRI agents for quantifying oxygenation in environments relevant to medical imaging .Objective Several studies have suggested that human cytomegalovirus (CMV) infection is closely related to the pathogenesis of atherosclerosis. The objective of this study is to investigate the association between human CMV infection and carotid atherosclerotic plaque vulnerability in Chinese population. Methods A total of 42 patients with carotid atherosclerosis (observation group) and 30 healthy volunteers (control group) were recruited in our study from October 2016 to January 2018. Statistical analysis was carried out to calculate the infection rate of CMV in subjects. Spearman's rank analysis was performed to evaluate the correlation between CMV infection and atherosclerotic plaque vulnerability. Results The positive rate of CMV was significantly higher in the observation group than in the control group, and matrix metalloproteinase 9 (MMP-9), tumor necrosis factor-α (TNF-α) and lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) expression levels were also elevated in the observation group compared with those in the control group. In carotid atherosclerotic patients, the detection rate of unstable plaques and Crouse scores in vulnerable plaque were significantly higher in CMV-positive group than those in the CMV-negative group. As revealed by correlation analysis, CMV infection was significantly positively correlated with plaque vulnerability and expression levels of MMP-9, TNF-α and LOX-1 in carotid atherosclerotic patients. Conclusion Human CMV infection might be a potential risk factor for increased plaque vulnerability in patients with carotid atherosclerosis.An adult dog was presented for chronic cough and a recent development of ulcerated, erythematous nares with nasal discharge. Cytology of enlarged peripheral lymph nodes revealed many intracellular and extracellular organisms. These round or rarely oval organisms measured approximately 5-9 µm in diameter and frequently contained several globular structures, ranging from deeply basophilic to magenta. A thin, clear halo was present. Smaller 1-2 µm, magenta forms were also observed. Fungal culture yielded small, wet, raised, irregularly shaped, white to pale tan colonies. Microbiologic staining of cultured material revealed features suggestive of algae. Histopathology of the lymph nodes revealed marked granulomatous inflammation with intralesional algal organisms suggestive of Prototheca. Electron microscopic findings were also consistent with protothecosis. Polymerase chain reaction, followed by direct DNA sequencing, identified the organism as Prototheca wickerhamii. A brief literature review discussing protothecosis in veterinary medicine is included.
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