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https://www.selleckchem.com/products/6-thio-dg.html We show that background fringe-pattern subtraction is a useful technique for removing static noise from off-axis holographic reconstructions and can enhance image contrast in volumetric reconstructions by an order of magnitude in the case for instruments with relatively stable fringes. We demonstrate the fundamental principle of this technique and introduce some practical considerations that must be made when implementing this scheme, such as quantifying fringe stability. This work also shows an experimental verification of the background fringe subtraction scheme using various biological samples.Sensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its widespread adoption. To overcome this limitation, we propose using wavelet analysis to quantify the loss of resolution due to the aberrations in microscope images. By examining the variations of the wavelet coefficients at different scales, we are able to establish a multi-valued image quality metric that can be successfully deployed in different microscopy techniques. To corroborate our arguments, we provide experimental verification of our method by performing aberration correction experiments in both confocal and STED microscopy using three different specimens.We report a chirped-pulse optical parametric oscillator (OPO) generating light pulses with an instantaneous-bandwidth much wider than the parametric gain-bandwidth of nonlinear crystals. Our numerical simulations show that a relatively high residual second-order-dispersion within the OPO cavity is required in order to achieve the maximum signal-bandwidth from an OPO system. Based on this principle, we constructed an OPO using a 3-mm-long PPLN crystal, which produced a signal wave with an instantaneous-bandwidth of 20 THz (at -20

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