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This field and abattoir study assessed the association of the severity and prevalence of small lungworm lesions with the carcass characteristics of 1332 lambs and adult sheep bred on three farms in southeast SA. Liveweight and measures of lungworm infection were measured on farm, then lung lesions and carcass characteristics assessed at slaughter. The overall prevalence of small lungworm lesions at slaughter was 79 % (928/1177; 95 % CI 76, 81), with a prevalence of 87 % (569/658; 95 % CI 84, 89) in lambs, and 69 % (359/519; 95 % CI 65, 73) in adults, respectively. Small lungworm infected lambs and adults had a similar hot standard carcass weight and dressing percentage compared to non-infected animals, both overall and within their respective cohort. Overall, the mean carcass weight for non-infected and infected lambs was 23.4 kg (95 % CI 18, 29), and 23.6 kg (95 % CI 18, 29), respectively, with a mean difference of 0.2 kg (95 % CI -0.4, 0.8; P = 0.5). Mean carcass weight for non-infected and infected adults was 21.3 kg (95 % CI 15, 28), and 21.5 kg (95 % CI 15, 28), with a mean difference of 0.2 kg (95 % CI -0.5, 0.9; P = 0.5). This study confirmed a very high prevalence of small lungworm lesions in sheep bred on farms in this region of SA, but their hot standard carcass weights were not reduced by these lesions. Additional information to compare the presence of lesions with productivity within an individual was collected at slaughter which provided more detailed information than is currently collected by routine abattoir surveillance. The limitations of the currently available diagnostic tests for small lungworm were also demonstrated. This indicated a need for the development of more sensitive tests to assess lungworm infections both on farm and at the abattoir. https://www.selleckchem.com/products/u18666a.html Currently, farmers in this region are concerned about the very high prevalence of small lungworm in their sheep, but this study provides reassurance that the presence of mild lesions does not reduce production.The management of equine strongyles has become problematic over the last decade because of an increased prevalence of drug-resistant isolates worldwide. Therapeutic options are therefore limited, leaving macrocyclic lactones as the most often effective drug class. However, their lipophilic properties result in a long-lasting elimination that could favour drug resistance selection. As a result, ivermectin treatment in lactating mares could promote suboptimal exposure of their foal parasites to ivermectin, thereby selecting for more resistant worms. To test for this putative transfer, we selected two groups of six foal-mare pairs, one group of mares receiving ivermectin and the other being left untreated. We compared faecal egg count trajectories in foals from the two groups and quantified plasma ivermectin concentrations in ivermectin treated mares and their foals during seven days. Our results showed limited but sustained plasmatic exposure of foals associated with non-significant faecal egg count reduction (P = 0.69). This suggests that ivermectin treatment in lactating mares results in suboptimal exposure to the drug in their foal. Induced pluripotent stem cells (iPSCs) have great potential as the basis of regenerative medicine. In this paper, we propose an automatic quality evaluation model based on multi-source feature ensemble learning to divide the iPSC colonies into three categories good, medium and bad. First, we obtained iPSCs samples using a Sendai virus reprogramming method. Second, we collected the bright field-images of iPSC colonies and processed them with adaptive gamma transform and data enhancement. The evaluation for the iPSC colony quality was further verified with living cell fluorescent staining, currently accepted as the optimal biological method. Third, multi-source features were extracted using three deep convolutional neural networks (DCNNs) and four traditional feature descriptors. Finally, we utilized a support vector machine (SVM) to perform classification. Before feeding into the SVM, the features were processed by principal component analysis algorithm to save computational cost and training time. Experimental results on the collected iPSC dataset (46,500 images) show that the proposed method could obtain 95.55% classification accuracy. Our study could provide a method to efficiently and quickly judge the biological quality of a single iPSC colony or populations and facilitate the large-scale iPSC manufacturing. Our study could provide a method to efficiently and quickly judge the biological quality of a single iPSC colony or populations and facilitate the large-scale iPSC manufacturing. The onset of maternal perinatal depression poses many challenges for fathers, yet in Singapore and Asia, the topic remains largely unexplored. This study aimed to gain insight into the experiences of fathers whose partners suffer from perinatal depression in the Asian milieu. A descriptive qualitative study design was adopted. Purposive and snowball sampling methods were used to recruit participants. Semi-structured, face-to-face interviews were conducted individually with participants to collect data, which were analysed using thematic analysis. Twelve fathers were recruited for the study. Eleven were from a perinatal mental health service in a tertiary hospital in Singapore, while 1 father was recruited via snowball sampling. Five themes describing the fathers' journey through their partners' perinatal depression emerged from the analysis. The themes are (1) feeling their world collapse; (2) struggling with the depression; (3) enduring the emotional toll; (4) coping with the situation; and (5) emerging from the other side. The findings of this study highlighted the struggles, coping methods, and support needs of fathers whose partners suffer from perinatal depression. Fathers were often unable to identify perinatal depression in their partners. Notably, they testified to the need to tolerate verbal and sometimes violent outbursts from their partners, and requested for more available information, resources, and peer support groups. The findings underscore a need for pre-emptive education on maternal perinatal depression and more accessible resources. Healthcare professionals should assess fathers' emotional responses to parenthood and their partners' depression. The findings underscore a need for pre-emptive education on maternal perinatal depression and more accessible resources. Healthcare professionals should assess fathers' emotional responses to parenthood and their partners' depression.
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