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https://www.selleckchem.com/products/amg-900.html In this paper, four types of middle-pressure chromatogram isolated gels are evaluated for adsorption/desorption characteristics of ginsenosides from Panax ginseng. Among them, SP207SS and SP2MGS were selected for dynamic investigations based on their static adsorption/desorption capacity of total ginsenoside. Their adsorption kinetics was better explained by pseudo-second-order model and isotherms were preferably fitted to Langmuir model. Dynamic breakthrough experiments indicated an optimum sample loading speed of 4BV/h for either SP207SS or SP2MGS. Desorption speed was determined to be 2BV/h according to desorption amount of total ginsenoside in their effluents. Eight ginsenosides were identified and quantified by HPLC-TQ-MS in total ginsenoside extract and different fractions during stepwise dynamic elution. For SP207SS, 27.62% of loaded ginsenosides was detected in 40% ethanol fraction, while 59.12% of them were found in 60% ethanol fraction. As on SP2MGS, the number went to 53.71% and 44.43% respectively. Recovery rate of ginsenosides were calculated to 78.65% for SP207SS and 89.53% for SP2MGS. Intriguingly, content of Rg1 and Re in 40% ethanol fraction from SP207SS became 20.1 and 18.6 times higher than that in total ginsenoside extract by one step elution, which could be leveraged for the facile enrichment of these two ginsenosides from natural sources. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.Thrombocytopenia-absent radius (TAR) syndrome is characterized by radial defect and neonatal thrombocytopenia. It is caused by biallelic variants of RBM8A gene (1q21.1) with the association of a null allele and a hypomorphic noncoding variant. RBM8A encodes Y14, a core protein of the exon junction complex involved in messenger RNA maturation. To date, only two hypomorphic variants have been identified. We report on a cohort of 26 patients aff

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