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https://www.selleckchem.com/products/Adriamycin.html Microscopy of the stained slides revealed that the biofilm has a layered structure. In each image obtained using a video eyepiece, it was possible to differentiate 4 layers. From the border of the two media to the inside the fragmentation layer, the dense layer, the matrix substance layer, and the last one - the persistence layer. Channels of different diameters (from 10 to 24 microns) are observed across the entire thickness of the biofilm. Thus, used approach allows us to visualize and evaluate the structure of microbial biofilm, measure the thickness of layers and channel diameters. In addition, this method can be used to study the effect of antimicrobial drugs on bacterial films.The expression of toll-like and adhesive receptors on epithelial cells of the oral mucosa changes in different pathological conditions, both local and systemic levels, in particular, in chronic periodontitis. The long-term presence of periodontal pathogenic microorganisms in the gingival furrow stimulates and supports the inflammatory process. The interaction of periodontal pathogens with epithelial cells of the oral mucosa is the first stage of the development of periodontitis. The pathological process affects the function of epithelial cells, in particular their ability to interact with representatives of microbiocenosis. Therefore, the natural colonization of normal oral microbiota on buccal epitheliocytes, reflecting the ability of epithelial cells to microbial adhesion, is a sensitive indicator of various destabilizing processes. Determining the level of expression of toll-like TLR2 and TLR4 receptors on epithelial cells also allows us to assess the functional state of cells and the severity of the inflan assessing the severity of the inflammatory process in chronic periodontitis than determining the level of natural colonization.Recent studies have shown that bacterial resistance existed long before antimicrobials were used in me

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