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This study could pave the way towards developing a convenient non-extraction aqueous phase analytical procedure for detection of FPs using Zn(II) complexation method.Actinoplanes sp. A1094 strain had been selected for its high production of acarbose from 20 different strains of Actinoplanes sp. can be found in wild. The content for glucosidase inhibitor of acarbose concentration was recorded at 1.12 g/L. The conducted analysis of 16S rRNA sequence of Actinoplanes sp. A1094 showed 99% similar identity to the corresponding sequence of Actinoplanes hulinensis. https://www.selleckchem.com/products/rimiducid-ap1903.html Acarbose was purified from Actinoplanes hulinensis 1094 with a yield of 8.48%, purity of 98% and further identified by LC/MS and NMR methods (C25H43NO18; m/z 645.6 g/mol). The purified acarbose was used to evaluate the hypoglycemia in streptozotocin (STZ)-induced diabetic mice model. The purified acarbose reduced postprandial blood glucose level in comparison with Glucobay® as medication for control type 2 diabetes in a combination therapy. Notably, the outcomes of native acarbose on fasting blood glucose levels in mice resemble akin to the commercial product and the acarbose accumulating fermentation and metabolic engineering from the cell gene in which would reduce in production cost. Therefore, acarbose from Actinoplanes hulinensis 1094 could be potentially used to make products for the treatment of type II diabetes.Co-occurrence of microplastics and chemicals was a complex environmental issue, whereas little concerned on the effect of microplastics on the toxicity of chemicals. In this study, we studied the changes of toxicity of triphenyl phosphate (TPhP) to zebrafish, in the presence of micro-polystyrene (MPS, 5.8 μm) and nano-polystyrene (NPS, 46 nm). Results indicated that separate MPS and NPS had no acute toxicity and little reproductive toxicity on zebrafish. TPhP alone was a highly toxic substance with a median lethal concentration (LC50) of 976 μg/L, the presence of MPS and NPS did not have significant effect on the acute toxicity of TPhP. TPhP alone stimulated the enlargement of liver and gonad of fish (except the testis) by 1.25-2.12 fold, and the presence of NPS further aggravated this stimulation by 1.23-2.84 fold, while MPS did not. The gonadal histology indicated that comparing with TPhP alone, the addition of MPS and NPS further inhibited the process of spermatogenesis and oogenesis, even causing obvious lacunas in testis and atretic follicles oocytes in ovary. Meanwhile, TPhP did not significantly disturb the hormone homeostasis (E2/T) and vitellogenin (Vtg) content in fish, but the presence of NPS increased the E2/T ratio and Vtg content in male fish, while slightly decreased those in female fish, which implying that effects of PS was sex-dependent. As a result, the egg production, the fertilization rate and hatchability of eggs significantly reduced after combined TPhP+PS exposure, demonstrating that co-occurrence of TPhP and PS, especially NPS, could greatly impaired the reproductive performance of zebrafish.Shifting to plant-based and low-carbon diets is a key measure for climate change mitigation. In this regard, national and local governments are setting goals and actions to tackle this issue. The municipality of Barcelona has set an intervention for the academic year 2020-21 introducing low-carbon meals in public schools. This study assesses the environmental and nutritional benefits of this intervention by applying the Life Cycle Assessment (LCA) methodology, with an energy and nutritional functional unit; and combined it with the Water-Energy-Food (WEF) nexus approach, by considering three WEF resources-based impacts (Blue Water Footprint (BWF), Primary Energy Demand (PED) and Land Use (LU)) and the Global Warming Potential (GWP). The transition to a low-carbon meal would reduce between 46 and 60% the environmental impacts. These benefits could even be higher when extra interventions within the school boundaries are applied. More research in behavioural change is needed in order to evaluate both the acceptance of the new menus by scholars and the adaptation of the school kitchen staff to the new menu. Finally, it is suggested to monitor the environmental and nutritional changes of the introduction of low-carbon meals within the school menus in an integrated way.Biomass is a type of renewable and sustainable resource that can be used to produce various fuels, chemicals, and materials. Nitrogen (N) in biomass such as microalgae should be reduced if it is used to produce fuels, while the retention of N is favorable if the biomass is processed to yield chemicals or materials with N-containing functional groups. The engineering of the removal and retention of N in hydrochar during hydrothermal carbonization (HTC) of biomass rich in protein is a research hot spot in the past decade. However, the N transformation during HTC has not yet been fully understood. In order to mediate the migration and transformation of N in hydrochar, the present review overviewed i) the characteristics of hydrochar and the original feedstock, ii) the possible N transformation behavior and mechanisms, and iii) the effect of factors such as feedstock and pyrolysis parameters such as temperature on hydrochar N. The high temperature and high protein content promote the dehydration, decarboxylation, and deamination of biomass to produce hydrochar solid fuel with reduced N content, while the Millard and Mannich reactions for lignocellulosic biomass rich in carbohydrate (cellulose, hemicellulose, and lignin) at medium temperatures (e.g., 180-240 °C) significantly promote the enrichment of N in hydrochar. The prediction models can be built based on properties of biomass and the processing parameters for the estimation of the yield and the content of N in hydrochar.Antimicrobial resistance (AR) represents a global threat in human and veterinary medicine. In that regard, AR proliferation and dissemination in agricultural soils after manure application raises concerns on the enrichment of endogenous soil bacterial population with allochthonous antibiotic resistance genes (ARGs). Natural resilience of agricultural soils and background concentrations of ARGs play key roles in the mitigation of AR propagation in natural environments. In the present study, we carried out a longitudinal sampling campaign for two crop vegetation periods to monitor spatial and temporal changes in the abundance of seven clinically relevant ARGs (sul1, ermB, vanA, aph(3')-IIa, aph(3')-IIIa, blaTEM-1 and tet(W)) and ribosomal 16S RNA. The absolute and relative abundances of the selected ARGs were quantified in total community DNA extracted from agricultural (manured and non-manured) and forest soils, fresh pig faeces and manure slurry. We observed that ARG concentrations return to background levels after manure-induced exposure within a crop growing season, highlighting the resilience capacity of soil.
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