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https://www.selleckchem.com/ Fascioliasis is a zoonotic infection of humans and, more commonly, ruminants. It is caused by 2 liver fluke species, Fasciola hepatica and Fasciola gigantica, which differ in size. The traditional morphological methods used to distinguish the 2 species can be unreliable, particularly in the presence of hybrids between the 2 species. The development of advanced molecular methods has allowed for more definitive identification of Fasciola species, including their hybrids. Hybrids are of concern, as it is thought that they could acquire advantageous traits such as increased pathogenicity and host range. In 2013, we collected flukes from Fasciola-positive cattle, sheep, and goats slaughtered in 4 Chadian abattoirs. DNA from 27 flukes was extracted, amplified, and analyzed to identify species using the ITS1+2 locus. Twenty-six of the 27 flukes were identified as F. gigantica, while the remaining fluke showed heterozygosity at all variable sites that distinguish F. hepatica and F. gigantica. Cloning and sequencing of both alleles confirmed the presence of 1 F. hepatica and 1 F. gigantica allele. To our knowledge, this is the first unambiguous, molecular demonstration of the presence of such a hybrid in a bovine in sub-Saharan Africa. © American Society of Parasitologists 2020.The purpose of this study was to describe the prevalence and incidence of Neospora caninum infection in dogs that are in close contact with dairy cattle and to identify possible risk factors associated with the infection in this population. Twenty-four dogs located in 8 different dairy farms of Aguascalientes, Mexico, were evaluated for a 6-mo period. Once a month a sample of serum and a sample of peripheral blood was collected. The serum was used to detect antibodies against N. caninum by means of the indirect immunofluorescence technique, and the blood was used to detect parasite's DNA. The association between seroprevalence and possible risk factors was estimated using

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