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https://www.selleckchem.com/products/sodium-palmitate.html In times of spreading multidrug-resistant bacteria, species identification and decontamination of cell cultures can be challenging. Here, we describe a mobile cell culture contaminant with "black dot"-like microscopic appearance in newly established irreplaceable hybridoma cell lines and its identification. Using 16S rRNA gene sequencing, species-specific PCRs, whole genome sequencing (WGS), and MALDI-TOF mass spectrometry, the contaminant was identified as the ubiquitous environmental and clinically relevant Gram-negative bacterium Ralstonia insidiosa (R. insidiosa), a strong biofilm producer. Further characterizations by transmission electron microscopy (TEM) and biochemical API test were not conclusive. Whole genome sequencing of our R. insidiosa isolate revealed numerous drug-resistance determinants. Genome-wide comparison to other Ralstonia species could not unambiguously designate our isolate to R. insidiosa ( less then 95% average nucleotide identity) suggesting a potential novel species or subspecies, closely related to R. insidiosa and R. pickettii. After determining the antibiotic susceptibility profile, the hybridoma cell culture was successfully decontaminated with ciprofloxacin without affecting antibody production.In this paper, hot gas pressure forming (HGPF) of Ti-55 high temperature titanium alloy was studied. The hot deformation behavior was studied by uniaxial tensile tests at temperatures ranging from 750 to 900 °C with strain rates ranging from 0.001 to 0.05 s-1, and the microstructure evolution during tensile tests was characterized by electron backscatter diffraction. Finite element (FE) simulation of HGPF was carried out to study the effect of axial feeding on thickness distribution. Forming tests were performed to validate this process for Ti-55 alloy. Results show that when the temperature was higher than 750 °C, the elongation was large enough for HGPF of Ti-55 alloy. Dynamic recry

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