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https://www.selleckchem.com/peptide/gsmtx4.html Although DDT, γ-HCH and PCBs have been almost completely withdrawn from world production and use, they are still present in the environment. Mushrooms are eagerly collected and consumed local raw material in north-eastern part of Poland. The aim of the study was to determine the content of DDT, γ-HCH and PCB residues in popular species (Boletus badius, Boletus edulis, Cantharellus cibarius) and to estimate the human exposure of uptaking of these compounds with mushrooms. The content of γ-HCH, DDT and PCB was determined using gas chromatography. The presence of analyzed compounds was observed in all the species under study. The highest content of γ-HCH and ΣDDT was determined in C. cibarius - 7.19 and 180.37 µg/kg of lipids, respectively, whereas the highest amount of ΣPCB was observed in B. edulis - 20.89 µg/kg of lipids. The contents were low and did not pose a threat to human health.Endo-fucoidanases are important in structural analysis of fucoidans and preparation of fuco-oligosaccharides. However their enzymological properties and analysis of degradation products are scarcely investigated. Truncated endo-α (1 → 3)-fucoidanase Fda1 (tFda1B from Alteromonas sp. was overexpressed and characterized, showing highest activity at pH 7.0, 35 °C, and 1.0 M NaCl. Its Km and kcat were 3.88 ± 0.81 mg/mL and 0.82 ± 0.17 min-1. Fe3+ and Mn2+ enhanced activity by 100% and 19.5% respectively. Co2+ and Cu2+ completely inactivated tFda1B, whereas Ni2+, Mg2+, Zn2+, Pb2+, Ca2+, Ba2+ and Li+ decreased activity by 58.8%, 56.0%, 50.6%, 47.7%, 28.9%, 15.6% and 37.5%, respectively. Catalytic residues were identified through structure and sequence alignment, and confirmed by mutagenesis. Degradation products of Kjellmaniella crassifolia fucoidan by tFda1B were characterized by LC-ESI-MS/MS, confirming tFda1B belongs to endo-(1 → 3)-fucoidanases, and backbone of K. crassifolia fucoidan is 1 → 3 fucoside linkage. This endo-α (1 → 3)-fucoidana

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