Yam Code

New Paste Browse

https://www.selleckchem.com/products/Cediranib.html The YeaZ protein of V. harveyi was expressed in Escherichia coli and purified. The purified recombinant protein YeaZ exhibited the protease activity. The proteolytic activities with azocasein as substrate were 39130 U mg-1 . The mutation of the amino acid in active sites such as Asp88 , Ser185 and Trp169 were performed. The enzyme activities of the purified mutant proteins with Asp88 -Ala, Ser185 -Leu and Trp169 -Glu were decreased to 24.28%, 35.27% and 41.66%, respectively. The mutant protein with two amino acid residues (Asp88 -Ala/Ser185 -Leu) lost the protease activity completely. Addition of the purified recombinant YeaZ increased resuscitation of the viable but non-culturable state (VBNC) cells to culturable state, and the culturable cell count increased from 1.35×102 cfu ml-1 to 3.10×106 cfu ml-1 . While addition of the mutant YeaZ without protease activities did not show obvious promoting effect on resuscitation of VBNC cells. Moreover, the purified YeaZ also showed lower muralytic activity, and the activities of proteins with single amino acids mutation (Thr71 and Asp112 ) were reduced from 7.05 U mg-1 to 4.75 U mg-1 and 2.50 U mg-1 , the resuscitation promoting effect on VBNC cells was not affected by these mutant proteins. These results implied that resuscitation promoting effect of YeaZ on VBNC cell was partly related to its protease activities, but not with the muralytic activity. This article is protected by copyright. All rights reserved.OBJECTIVES The objectives of this study were 1) to assess the prevalence of ultrasound features of adenomyosis in an infertile population undergoing in vitro fertilization (IVF), 2) to define the inter- and intra-rater agreement in three-dimensional ultrasound (3D US) assessment of adenomyosis, and 3) to evaluate sonographic features of adenomyosis with respect to pregnancy outcomes following transfer of a single thawed euploid blastocyst. METHODS A prospective cohor

Paste Settings

Paste Title : [Optional]

Paste Folder : [Optional]

Syntax Highlighting : [Optional]

Paste Expiration : [Optional]

Paste Status : [Optional]

Password : [Optional]

Description: [Optional]

Tags: [Optional]

Encrypt Paste (?)

You are currently not logged in, this means you can not edit or delete anything you paste. Sign Up or Login

Paste Settings

Site Languages