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https://www.selleckchem.com/products/gsk-3484862.html However, sequence analysis of MfCYP51 from 25 DMI-resistant isolates did not reveal any point mutations that could be correlated with resistance. Amplification and sequencing upstream of MfCYP51 resulted in detection of DNA insertions in a wide range of isolates typed by DMI phenotype and the presence of Mona or other unique sequences. The function of these unique sequences or their presence upstream of MfCYP51 cannot be correlated to a DMI-resistant genotype at this time. Our results indicate that DMI resistance was established in Michigan populations of M. fructicola by 2009 to 2011, and that relative resistance levels have continued to increase to the point that practical resistance is present in most orchards. In addition, the presence of the Mona insert is not a marker for identifying DMI-resistant isolates of M. fructicola in Michigan.Blackberries (Rubus L. subgenus Rubus Watson) are popular, wild fruits with high content of antioxidants and thus with beneficial effect on the human health (Reyes-Carmona et al. 2005). In July 2019 and May 2020, plants with typical powdery mildew symptoms were collected in the blackberry cv. 'Triple crown' orchard (of 2 ha in size) in the vicinity of Pakovraće (Moravica District, Serbia). The symptoms observed in 2019 included mild chlorotic spots on both old and young leaves accompanied by the white powdery mildew colonies on the surface of the leaves, visible on both primorcanes and floricanes. In 2020, even more intensive symptoms occurred on fruit bearing shoots which were covered with dense white fungal growth. Heavily infected leaves turned necrotic along the edges, followed by defoliation. Disease incidence was calculated by randomly counting and rating 100 plants in four replications and estimated to be over 90% while disease severity was estimated to be over 40%. Morphological characteristics were ae. P. aphanis is described as strawberry and raspberry powdery mildew

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