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dry surface biofilms (DSBs) that offer a potential reservoir for transferable pathogens. While the role of keyboards in transmission is uncertain, there is a need to pursue effective solutions for eliminating DSBs from keyboards. During the SARS-CoV-2 pandemic, healthcare workers (HCWs) are being exposed to infection both at work and in their communities. Determining where HCWs might have been infected is challenging based on epidemiological data alone. At Akershus University Hospital, Norway, several clusters of possible intra-hospital SARS-CoV-2 transmission were identified based on routine contact tracing. To determine whether clusters of suspected intra-hospital SARS-CoV-2 transmission could be resolved by combining whole genome sequencing (WGS) of SARS-CoV-2 with contact tracing data. Epidemiological data were collected during routine contact tracing of polymerase chain reaction-confirmed SARS-CoV-2-positive HCWs. Possible outbreaks were identified as wards with two or more infected HCWs defined as close contacts who tested positive for SARS-CoV-2 less than three weeks apart. Viral RNA from naso-/oropharyngeal samples underwent nanopore sequencing in direct compliance to the ARTIC Network protocol. Five outbreaks were suspected from contact tracing. Viral consensus sequences from 24 HCWs, two patients, and seven anonymous samples were analysed. Two outbreaks were confirmed, one refuted, and two remained undetermined. One new potential outbreak was discovered. Combined with epidemiological data, nanopore WGS was a useful tool for investigating intra-hospital SARS-CoV-2 transmission. WGS helped to resolve questions about possible outbreaks and to guide local infection prevention and control measures. Combined with epidemiological data, nanopore WGS was a useful tool for investigating intra-hospital SARS-CoV-2 transmission. WGS helped to resolve questions about possible outbreaks and to guide local infection prevention and control measures. To investigate the effect of scanned area on the accuracy and scan time of intraoral scans of an anterior implant. Three operators experienced in intraoral scanning (at least 2-year experience) performed partial and complete-arch scans (n = 10) of a dentate resin model with an implant at left central incisor site by using an intraoral scanner (Trios3; 3Shape, Copenhagen, Denmark). Each partial- or complete-arch scan was superimposed to a reference scan from a laboratory scanner (Ceramill Map 600; Amann Girrbach AG). Mean distance (selected 7 points) and angular (mesiodistal and buccolingual) scanbody deviations in test scans (trueness) and their variance (precision) were calculated. Linear-regressions (trueness), two-sided F-tests with a Bonferroni correction (precision), and multiple linear regressions (scan time), with the operator as a covariate were applied (alpha = .05). Interactions were found between the scanned area and the operator for their effect on trueness of all points and angles, except for point 6 at implant-abutment connection in mesiodistal plane (p < .05). No significant difference was found between the precision of partial and complete-arch scans for all operators (p > .05). Partial-arch scan times were significantly shorter, overall, and for each operator (p < .001). No significant effect of scan time was found on the trueness of partial- and complete-arch scans (p > .05). Partial and complete-arch scans of anterior single implants with an intraoral scanner resulted in similar accuracies, and were not influenced by the operator or the scan time. Scan times of partial-arch scans were significantly shorter. Partial-arch scans can be used for the fabrication of monolithic anterior single implant crowns because the scans can be completed in shorter times without compromising the accuracy. Partial-arch scans can be used for the fabrication of monolithic anterior single implant crowns because the scans can be completed in shorter times without compromising the accuracy. To determine the efficacy of a cosmetic aluminium lactate/potassium nitrate/hydroxylapatite toothpaste for the reduction of dentine hypersensitivity (DH) pain as compared to a control toothpaste containing potassium nitrate. The study was a randomised, examiner-blind, two treatment arm, parallel controlled trial in healthy adults with at least 2 sensitive teeth (Schiff >2). At baseline, immediately after treatment and at 7 and 14 days of twice-daily brushing of the test or control toothpaste the sensitivity of 2 test teeth was measured following iced-water (Schiff and VAS) and tactile (Yeaple probe) stimuli, and a whole mouth plaque score was obtained. Participants also completed a whole-mouth VAS and DHEQ15 quality of life questionnaire at baseline, 7 and 14 days. Both toothpastes reduced DH in test teeth, but pain reduction in the test group was significantly better at all timepoints and by all measures (p = 0.005, tooth-level VAS immediately after brushing; p < 0.001 all other comparisons). There, is arresting in magnitude, affecting quality of life. Daily application of efficacious toothpastes can relieve DH pain however, as yet, there is no gold standard treatment. The results of this study support further investigation of an aluminium lactate/potassium nitrate/hydroxylapatite toothpaste for DH management. Inflammation can lead to hyperalgesia and allodynia by activation or sensitization of peripheral and central nervous system neurons. This study aimed to assess the occurrence of secondary thermal hyperalgesia in patients with symptomatic irreversible pulpitis (SIP). The cold sensitivity test (visual analog scale) was performed for the tooth with SIP, its adjacent sound tooth, the same sound tooth in the opposite jaw, and the contralateral sound tooth in the opposite quadrant of the same jaw. Next, the tooth with SIP underwent root canal treatment, and 3 weeks later, after complete elimination of pain, the teeth underwent cold sensitivity testing again. A total of 64 patients, including 41 women and 23 men 18-65 years old, were evaluated in this study. https://www.selleckchem.com/products/trastuzumab-deruxtecan.html The response to the cold sensitivity test significantly decreased in the tooth with SIP (P < .001), its adjacent sound tooth (P < .001), and the same sound tooth in the opposite jaw (P = .004) but not in the contralateral sound tooth in the opposite quadrant of the same jaw (P = .
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