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https://www.selleckchem.com/products/as601245.html Here, we identified genes that influenced Escherichia coli fitness during cooperative growth with a mutualistic partner, Rhodopseudomonas palustris Although this mutualism centers on the bidirectional exchange of essential carbon and nitrogen, E. coli fitness was positively and negatively affected by genes involved in diverse cellular processes. Furthermore, we discovered an unexpected purine cross-feeding interaction. These results contribute knowledge on the genetic foundation of a microbial cross-feeding interaction and highlight that unanticipated interactions can occur even within engineered microbial communities. Copyright © 2020 American Society for Microbiology.Microorganisms in nature are commonly exposed to various stresses in parallel. The isiA gene encodes an iron stress-induced chlorophyll-binding protein which is significantly induced under iron starvation and oxidative stress. Acclimation of oxidative stress and iron deficiency was investigated using a regulatory mutant of the Synechocystis sp. PCC 6803. In this study, the ΔisiA mutant grew more slowly in oxidative stress and iron depletion conditions compared to the wild type (WT) counterpart under the same conditions. Thus, we performed RNA-seq analysis of WT and ΔisiA under double stress conditions to obtain a comprehensive view of isiA-regulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed significant differences between WT and ΔisiA, mainly related to photosynthesis and the iron-sulfur cluster. The deletion of isiA affects the expression of various genes that are involved in cellular processes and structures, such as photosynthesis, phycobilisomen which the blue module was correlated with oxidative stress. We further demonstrated that the isi operon contained the following five genes isiA, isiB, isiC, ssl0461, and dfp by co-transcriptional PCR. Three sRNAs were identified that were relate

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