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https://www.selleckchem.com/products/muramyl-dipeptide.html Bulk fertilizer application is one of the easiest means of improving yield of crops however it comes with several environmental impediments and consumer health menace. In the wake of this situation, sustainable agricultural practices stand as pertinent agronomic tool to increase yield and ensure sufficient food supply from farm to fork. In the present study, efficacy of iron-pulsing in improving the rice yield has been elucidated. This technique involves seed treatment with different concentrations (2.5, 5 and 10 mM) of iron salts (FeCl3 and FeSO4) during germination. FeCl3 or FeSO4 was used to treat the sets and depending on the concentration of the salts, the sets were named as C2.5, C5, C10 and S2.5, S5, S10 (where C and S stands for FeCl3 and FeSO4 respectively and the numbers succeeding them denotes the concentration of salt in mM). Our investigation identified 72 h of treatment as ideal duration for iron-pulsing. At this time point, the seedling emergence attributes and activities of α-amylase and protease increased. The relative water uptake of the seeds also increased through upregulation of aquaporin expression. The treatment efficiently maintained the ROS balance with the aid of antioxidant enzymes and increased the iron content within the treated seeds. After transplantation in field, photosynthetic rate and chlorophyll content enhanced in the treated plants. Finally, the post-harvest agro-morphological traits (represented through panicle morphology, 1000 seed weight, harvest index) and yield showed significant improvement with treatment. Sets C5 and S5 showed optimum efficiency in terms of yield improvement. To our best knowledge, this study is the first report deciphering the efficacy of iron-pulsing as a safe, cost effective and promising technique to escalate the yield of rice crops without incurring an environmental cost. Thus, iron-pulsing is expected to serve as a potential tool to address

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