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https://www.selleckchem.com/products/1-deoxynojirimycin.html The coupling between neuronal activity and cerebral blood flow (CBF), known as neurovascular coupling, has been reported to be impaired after stroke. This study aims to investigate the neurovascular coupling impairment at the acute stage after ischemic stroke. Laser speckle contrast imaging (LSCI) was applied to measure the hemodynamic response to optogenetic excitation of sensorimotor neurons in healthy and ischemic brain. The results showed that the hemodynamic response to optogenetic stimulation decreased and the regional CBF response was correlated with the distance from the ischemic core at the acute stage, regardless of the change in resting CBF. Our results also demonstrated that excitatory neuronal stimulation of intact area could promote the recovery of neurovascular coupling, whereas peri-infarct neuronal excitation failed to restore neurovascular function 24 hrs after ischemia. These results suggested the intact periphery of penumbra as the target for excitatory stimulation in aspect of restoring the perfusion after ischemic stroke.Platelet and fibrin-rich blood clots can respond differently to red blood cell rich clots during ischemic stroke treatment, which includes thrombolysis and mechanical thrombectomy. Currently, there is no accurate way to identify the type of clot in advance of treatment. If the type of blood clot can be identified, the optimum clot removal process can be chosen and patient outcomes can be improved. In this paper we fabricate physiologically relevant blood clot analogues from human blood, that cover a range of red blood cell, fibrin, and platelet concentrations. We characterize the dielectric profile of these formed clots using an open-ended coaxial probe method across a wide frequency range. After the dielectric measurements are completed, histology on each blood clot is performed to determine the concentration of red blood cells present. In total, 32 unique blood clot

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