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https://www.selleckchem.com/products/pf-3758309.html Transfection of miR-205 mimic or inhibitor was performed to alter the levels of miR-205 in astrocytes followed by western blot to assess Kir4.1 channel levels in these cells. Astrocytes treated with miR-205 mimic had a 38.6% reduction of Kir4.1 protein levels compared to control (mock-transfected) cells. In contrast, astrocytes transfected with miR-205 inhibitor were significantly upregulated compared to mock by 47.4%. Taken together, our data indicate that miR-205 negatively regulates the expression of Kir4.1 in astrocytes grown in hyperglycemic conditions.BACKGROUND Anastomotic leakage remains a dreaded complication after colorectal surgery. Stem-cell-based therapies have been shown to increase angiogenesis and cell proliferation. OBJECTIVE To investigate the use of adipose-derived stem cells on the healing of ischemic colonic anastomoses in a rat model. DESIGN This is an animal research study using xenotransplantation. SETTINGS Male Wistar rats (300-400g, n=48) were purchased from a licensed breeder. PATIENTS Adipose stem cells were isolated from the subcutaneous fat of healthy human donors. INTERVENTIONS The rats underwent laparotomy with creation of an ischemic colorectal anastomosis created by ligation of mesenteric vessels. The animals were divided into three groups control group with an ischemic anastomosis, vehicle only group in which the ischemic anastomosis was treated with an absorbable gelatin sponge, and a treatment group in which the ischemic anastomosis was treated with an absorbable gelatin sponge plus adipose stem cells. Animals were sacrificed reducing the risk of anastomotic leakage in colorectal surgery. See Video Abstract at http//links.lww.com/DCR/B203.BACKGROUND Most hospitals in the United States are reimbursed for colectomy via a bundled payment based on the diagnosis-related group assigned. Enhanced recovery after surgery programs have been shown to improve the value of colorectal surger

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