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https://www.selleckchem.com/products/agk2.html 39-20.96%, 0.03-7.95%, and 0.39-14.15% in Pw1 and 0.23-15.68%, 0.01-15.68%, and 0.53-26.77% in Cd1, respectively. The adsorption/uptake was significantly correlated with the perfluoroalkyl chain length (p less then 0.05), except for the uptake of biofilms in C. demersum. Furthermore, PFAAs and submerged macrophytes could decrease the richness of microbiota but increase the relative abundance of some strains in Betaproteobacteriales, Sphingomonadales, and Cytophagales. Our results were helpful for understanding the removal processes of PFAAs in constructed wetlands and their linkages with PFAA properties, thus further providing insight into the management and removal of emerging organic contaminants.Engineered nanoparticles are observed to be released into the environment and ended up in wastewater treatment plants. It has been reported that these nanoparticles in sewage might have a toxic effect on microorganisms, and thus affect anaerobic microbial fermentation. However, the mechanisms involved in nanoparticles-induced effects on the anaerobic acidification process and its related bacterial metabolism are still unclear. This work indicated that copper nanoparticles (Cu NPs) were able to cause cell membrane oxidative damage and inhibit the growth and metabolism of Moorella thermoacetica (a model acetogen). The OD600 and acetic acid production of M. thermoacetica in the presence of 1 mg/L of Cu NPs were decreased to 29.2% and 40.7% of the control, respectively. The key mechanism of the inhibitory effect was governed by the fact that Cu NPs significantly reduced the glucose consumption, and led to the decreased pyruvate metabolism levels. Additionally, Cu NPs inhibited the gene expressions and catalytic activities of the key enzymes related to acetic acid production. It was identified that the relative activities of phosphofructokinase, pyruvate kinase, phosphotransacetylase, and acetate kinase of M. thermoacetica in the

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