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https://www.selleckchem.com/products/ab928.html The inhibition of denitrification by heavy metals is a problem in nitrogen wastewater treatment, but the solutions are rarely studied. In this study, Pseudomonas brassicacearum LZ-4, immobilized in sodium alginate-kaolin, was applied in an activated-sludge reactor to protect denitrifiers from hexavalent chromium (Cr(VI)). Q-PCR result showed that the strain LZ-4 was incorporated into activated sludge under the help of immobilization. In the non-bioaugmentation system, the removal efficiency of nitrate was decreased by 86.07% by 30 mg/L Cr(VI). Whereas, denitrification was protected and 95% of nitrate was removed continuously in immobilized-cell bioaugmentation system. Miseq sequencing data showed that bioaugmentation decreased the impact of Cr(VI) on microbial communities and increased the abundance of denitrifiers. Based on the results of biomass and extracellular polymers, activated sludge was protected from Cr(VI) toxicity. This discovery will provide a feasible technique for nitrogen wastewater treatment in the presence of distressing heavy metals. A single helical baffle (SHB), consisting of twisted turns, was developed to convert straight flow into spiral flow in a Chlorella PY-ZU1 open raceway pond (ORWP) bubbled with 15% CO2. Microalgal solution flowing through the SHB alternative helical interspaces generated whirling flow both vertically and horizontally, which decreased mixing and increased mass transfer rates. The optimized SHB had a pitch length to total SHB length ratio of 0.13 and SHB diameter to ORWP single channel width ratio of 0.30, which decreased mixing times and increased mass transfer coefficients by 41.1% and 38.4% respectively. SHB moved Chlorella PY-ZU1 from the ORWP bottom to the top, increasing light exposure for photosynthesis. Cellular electron transfer rates and photochemical efficiency (φPSII) increased by 18%, chlorophyll a content increased by 16% and variable to maximum fluorescence r

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