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Copper nanoparticles were synthesized using Manilkara zapota leaf extract. The synthesis of the nanoparticle was primarily visualized when the colour of the reaction mixture turned into reddish-brown. Biosynthesized nanoparticles were characterized by UV-vis, FT-IR, XRD, SEM and EDX. The UV spectra showed maximum absorption at 584 nm. FT-IR studies showed stretching frequency at 592.76 cm-1, which is the fingerprint region for Cu-O bond. The crystallinity of the synthesized copper nanoparticles (Mz-Cu NPs) was revealed through XRD analysis. The synthesized Mz-Cu NPs were spherical with an average size of 18.9-42.5 nm and it was shown by SEM analysis. EDX analysis displayed that the nano sample contains 58 % of copper. The antimicrobial property of the synthesized nanoparticles was evaluated against fungal plant pathogens Rhizoctonia solani (MTCC 12232), Sclerotium oryzae (MTCC 12230) and bacterial species, namely Bacillus subtilis (ATCC 23857), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Vibrio harveyi (ATCC 35084), Vibrio parahaemolyticus (ATCC 33845). In in-vitro haemolytic assay, the particle showed 5.73, 3.34, 0.5 % hemolysis at 100, 50, 25 μg/mL concentration respectively. In the antiproliferative assay, the IC50 values of MCF7 and Vero cells were found to be 53.89 and 883.69 μg/μl. The particle degraded Methyl violet, Malachite green and Coomassie brilliant blue by 92.2, 94.9 and 78.8 %, within 50, 40 and 60 min, respectively, through its photocatalytic activity. To evaluate retinal thickness, area of foveal avascular zone (FAZ), flow area and flow density of choriocapillaris, vessel density of both superficial capillary plexus (SCP) and deep capillary plexus (DCP) of eyes with adult-onset foveomacular vitelliform dystrophy (AOFVD) using optical coherence tomography angiography (OCT-A) and compare the results with healthy controls. 17 eyes of 14 patients diagnosed with AOFVD and 17 eyes of 17 healthy subjects were enrolled in this study. All patients underwent a complete ophthalmological examination and a 6 x 6 mm macular OCT-A scanning. Quantiative results of retinal thickness, retinal vessel density of SCP and DCP, FAZ area, flow area and flow density of choriocapillaris were analyzed. No statistically significant differences were noted in the vessel density of the SCP, except for the parafoveal nasal sector (P = 0.048). Similarly, no statistically significant differences were observed in the vessel density of the DCP, except for the parafoveal (P = 0.037) and the parafoveal temporal (P = 0.048) sectors. The choriocapillaris flow area and the flow density were significantly lower in the patients with AOFVD than in the healthy controls (P = 0.001 for both). The mean FAZ area and the retinal thickness measurements were comparable in both groups. Patients with AOFVD show vascular abnormalities that can be detected with OCT-A. OCT-A, as a noninvasive imaging modality, could provide a new perspective for understanding the pathophysiology of AOFVD and could also be useful in the follow-up of these patients and in the management of the disease progression. Patients with AOFVD show vascular abnormalities that can be detected with OCT-A. OCT-A, as a noninvasive imaging modality, could provide a new perspective for understanding the pathophysiology of AOFVD and could also be useful in the follow-up of these patients and in the management of the disease progression. Little is known about how the chemical composition of enamel affects the optical properties of teeth, but advances in technology allow this to be studied using white-light and fluorescent images. This study aimed to identify the variation in enamel chemical composition that may affect tooth optical properties, such as tooth color and autofluorescence. Sixty-one specimens of extracted human molars were prepared. Raman spectrometry was used to assess chemical composition of enamel, and tooth color, and autofluorescence from enamel were evaluated by quantitative light-induced fluorescence (QLF) images. Pearson correlation and multiple linear regression analyses were used. Enamel fluorescence was related to enamel composition rather than tooth color. The b* value from the fluorescence image had a moderate correlation with crystallinity (full-width at half-maximum r = -0.433, p < 0.001) and laser-induced fluorescence intensity (r = 0.450, p < 0.001) from Raman spectroscopy. In multiple linear regressiohetic restorative materials. Photodynamic therapy (PDT) is a bactericidal method, which has recently been introduced in the field of dental medicine and therapy. Curcumin, a compound isolated from Curcuma longa L., exerts potent phototoxic effects at micromolar concentrations. The aim of our study was to explore the in vitro antimicrobial effect of curcumin-based PDT on two major etiological agents of periodontitis, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. P. gingivalis ATCC33277 and A. actinomycetemcomitans ATCC24523 suspension was irradiated with blue LED (BL) (450-470 nm, output power density 1.2 W/cm ) for 20-60 s (6-18 J/cm ), treated with curcumin (2 min), or subjected to a combination of curcumin treatment and BL irradiation (60 s, 18 J/cm ). A suspension of chlorhexidine gluconate (CHG) was used as the positive control. All bacterial suspensions used were serially diluted, plated, and incubated anaerobically or microaerobically. The numbers of colony-forming units (CFUs) were counted on day 7. Onne on P. gingivalis and A. actinomycetemcomitans were not significant, A. https://www.selleckchem.com/products/filanesib.html actinomycetemcomitans was susceptible to photodynamic inactivation by the combination of BL and curcumin. The aim of the present clinical trial was to evaluate the efficacy of antimicrobial photodynamic therapy (PDT) for the treatment of halitosis in elderly patients wearing dentures. Elderly patients (>60 years age) wearing complete dentures and diagnosed with halitosis (H S levels >112 ppb) were divided into two groups patients undergoing treatment with tongue scraper and full mouth disinfection (Group I) and patients undergoing treatment with tongue scraper, full mouth disinfection and single application of PDT (Group II). Oral health related quality of life (OHRQoL) was assessed using a 14-point Likert scale used in oral health impact profile (OHIP). The assessment of halitosis was done with the help of a device called Oral Chroma™. Microbial sampling for detection of Porphyromonas gingivalis (P. gingivalis) was performed from the dorsum of the tongue using a sterile swab at baseline and after treatment on day 5, 15 and 30 and quantified using polymerase chain reaction. Forty elderly patients with halitosis completed the trial.
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