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https://www.selleckchem.com/products/n-ethylmaleimide-nem.html 5. The Inference of this study that there is an increase in SGOT and SGPT levels in patients who consume OAT 1-2 months in RSUD Kota Kendari. Elevated levels of SGPT and SGOT due to Anti Tuberculosis (OAT) drugs did not occur in all patients and only increased in 3 people, but qualitatively the effect of administering anti-tuberculosis drugs significantly increased levels of SGPT and SGPT. Thus, monitoring of liver physiology remains recommended especially for elderly patients.Biofilms are able to cause microorganisms to be 80% more resistant to antibiotics. The extracelullar polymeric substance (EPS) in biofilm functions to protect bacteria, making it difficult for antibiotics to penetrate the biofilm layer. This study aims to determine the effectiveness of photodynamic inactivation with blue diode laser to reduce Staphylococcus aureus biofilm at various ages of biofilms. The light source is a 403 nm blue diode laser with an energy power of about 27.65±0.01 mW. The study was designed with two groups Group C was the untreated control group with variations in age of biofilms (0; 6; 11; 17; 24; 32; 40 and 48) hours; Group T was a laser treatment group with variations in age of biofilm and energy density (4.23; 8.46; 12.70; 16.93 and 21.16) J/cm2. Biofilm reduction measurement method using ELISA test was performed to calculate OD595 value. The statistical analysis results of variance showed that there was an influence of biofilm age and irradiation energy density of laser on biofilm reduction. Optical density analysis showed the most optimum biofilm reduction happened when biofilm age is perfectly constructed (about 17 hours) and with 91% reduction. The longer biofilm age lived among those biofilms, the greater the reduction. The results of the Scanning Microscope Electron and fluorescent microscope measurement showed destruction site of the EPS biofilm and bacterial cell death. So, the activated photodynam

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