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https://www.selleckchem.com/products/gdc-0068.html To establish a nucleic acid assay for detection of based on recombinase-aided isothermal amplification (RAA) assay. The gene of was selected as the target gene, and the specific primers and fluorescent probes for RAA assay were designed, screened and synthesized to establish a fluorescent RAA assay for detection of . The sensitivity of the fluorescent RAA assay was evaluated using different copy numbers of target gene sequence-contained recombinant plasmids and various concentrations of genomic DNA as templates, and the specificity of the fluorescent RAA assay was evaluated using the genomic DNA from , , , , , , , and as templates. A fluorescent RAA assay was successfully established for detection of , which achieved specific amplification of genomic DNA within 20 min at 39 ℃. The lowest detection limit of the fluorescent RAA assay was 10 copies/μL of recombinant plasmids and 0.1 ng/μL genomic DNA, which exhibited a high sensitivity, and the fluorescent RAA assay was all negative for the genomic DNA from , , , , , , and , which exhibited a high specificity. In addition, this fluorescent RAA assay successfully detected genomic DNA from cysts. A rapid, sensitive and specific fluorescent RAA assay is successfully established for nucleic acid detection of . A rapid, sensitive and specific fluorescent RAA assay is successfully established for nucleic acid detection of E. granulosus. To establish a recombinase-aided isothermal amplification (RAA) assay for nucleic acid detection of . The 121 bp highly-repeated sequence of was selected as the target gene fragment to be detected. The primers and fluorescent probes were designed using the Amplfix software, and a fluorescent RAA assay was established and optimized. The fluorescent RAA assay was performed to detect gradient diluent recombinant plasmids containing target gene fragment and different concentrations of genomic DNA to determine the sensitivity, and this assay was a

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