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https://www.selleckchem.com/EGFR(HER).html This study aimed to carry out an exhaustive chemical characterization of citrus (orange, mandarin, and grapefruit) decoctions as well as the residues obtained during the decoction process (by-products). The citrus decoctions were rich in hesperidin, naringin, and narirutin, but carotenoids and phytosterols were not detected. Interestingly, these flavanones were found in a higher concentration in the extractable polyphenol fraction of the citrus decoction by-products. Moreover, the greatest content of hesperidin and naringin was found bound to the food matrix by ether/ester bonds. Violaxanthin and β-cryptoxanthin were found as major carotenoids in the orange and mandarin decoction by-products, respectively, whereas the grapefruit decoction by-product showed a low content of carotenoids. All citrus by-products showed β-sitosterol as the major phytosterol, followed by β-campesterol. Therefore, the by-products obtained during the elaboration of citrus decoctions are rich sources of bioactive compounds that can be used for the development of functional foods or dietary supplements.During winemaking a wide variety of processing aids such as albumin, caseinates and lysozyme are often used. These proteins are considered allergenic and could become a human health risk in susceptible individuals. In our knowledge, there are no methods published for the analysis of these three proteins simultaneously by liquid chromatography with tandem mass spectrometry, with electrospray ionization. Therefore, in this work, a sample preparation approach for the analysis of α-casein, β-casein, albumin and lysozyme, in a single run, was performed and compared with published data. Through a pH adjustment, combining the use of cellulose ester membranes, a precipitation with organic solvents and a final concentration/clean-up, we achieved recovery values from 90.7 to 108.6%. The method was validated, showing determination coefficients R2 ≥ 0.99. This met
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