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https://www.selleckchem.com/products/cwi1-2-hydrochloride.html Formation of groups in variables can be applied to many case studies in order to have a clear idea of our data and the relevance of each of them in our dependent one. Fuzzy linear regression can be used in order to check the final results and ensure that the equivalence relations are a such a good method used in the data analysis while the researchers save time in long procedures of analyzing parameters not very close involved to the phenomenon investigated every time. Amphotericin B is a crucial agent in the management of serious systemic fungal infections. It is also known to be cytotoxic. In this study, we evaluated the effect of amphotericin B added to the preservation solution on islet yield during islet isolation. Porcine pancreata were preserved in the preservation solution with or without amphotericin B (0.25μg/mL) for approximately 18hours at 4°C, and then islet isolation was performed. An optimized number (1750IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice. The culture of isolated islets and acinar tissue with amphotericin B was also evaluated. The islet yield before and after purification in the amphotericin B (-) group was significantly higher than that in the amphotericin B (+) group. After islet transplantation into diabetic mice, blood glucose levels reached the normoglycemic range, with 50% and 0% of that of the diabetic mice in the amphotericin B (-) and amphotericin B (+) groups, respectively. In the culture study, amphotericin B was found to be cytotoxic to porcine islets and acinar tissue. Amphotericin B added to the preservation solution deteriorates islet yield during porcine islet isolation. Thus, the use of amphotericin B should be considered carefully for the preservation of the pancreas for islet isolation and islet culture before islet transplantation. Amphotericin B added to the preservation solution deteriorates islet yield

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