CITED2 is a transcription co-activator that interacts with TFAP2 and CBP/ P300 transcription factors to regulate the proliferation and differentiation of the cardiac progenitor cells. It acts upstream to NODAL-PITX2 pathways and regulates the left-right asymmetry. Both human genetic and model organism studies have shown that altered expression of CITED2 causes various forms of congenital heart disease. Therefore, we sought to screen the coding region of CITED2 to identify rare genetic variants and assess their impact on the structure and function of the protein. Here, we have screened 271 non-syndromic, sporadic CHD cases by Sanger's sequencing method and detected a non-synonymous variant (c.301C>T, p.P101S) and two synonymous variants (c.21C>A, p.A7A; c.627C>G, p.P209P). The non-synonymous variant c.301C>T (rs201639244) is a rare variant with a minor allele frequency of 0.00011 in the gnomAD browser and 0.0018 in the present study. in vitro analysis has demonstrated that p.P101S mutation upregulates the exprbuting to pathogenesis of CHD.
  Frailty syndrome is an established predictor of adverse outcomes after surgical procedures. Our study aimed to compare the simplified National Surgical Quality Improvement Program 5-factor-modified frailty index (mFI-5) to its prior 11-factor-modified frailty index (mFI-11) with respect to the predictive ability for mortality, postoperative complications, and unplanned 30-d readmission in patients undergoing lower limb amputation.
 
  The National Surgical Quality Improvement Program (2005-2012) databank was queried for all geriatric patients (>65 y) who underwent above-knee and below-knee amputations. We calculated each mFI by dividing the number of factors present for a patient by the total number of available factors. To assess the correlation between the mFI-5 and mFI-11, we used Spearman's rho rank coefficient. We then compared the two indices for each outcome (30-d complication, 30-d mortality, and 30-d readmission) and C-Statistic using predictive models.
 
  A total of 8681 patients were included with mean age of 76±9y, complication rate 35.8%, mortality rate 10.2%, and readmission rate 15.9%. There was no difference in type of amputation in frail and nonfrail. Correlation between the mFI-5 and mFI-11 was above 0.9 for all outcome measures. Both mFI-5 and mFI-11 indexes had strong predictive ability for mortality, postoperative complications, and 30-d readmissions.
 
  In patients undergoing major lower limb amputation, we found mFI-5 and the mFI-11 were equally effective in predicting postoperative outcomes. Frailty remained a strong predictor of postoperative complications, mortality, and 30-d readmission.
 In patients undergoing major lower limb amputation, we found mFI-5 and the mFI-11 were equally effective in predicting postoperative outcomes. Frailty remained a strong predictor of postoperative complications, mortality, and 30-d readmission.Stereoisomeric determination of individual triacylglycerols (TAGs) in natural oils and fats is a challenge due to similar physicochemical properties of TAGs with different fatty acid combinations.  https://www.selleckchem.com/products/ms023.html  In this study, we present a strategy to resolve the enantiomeric composition of nutritionally important TAGs in sea buckthorn (Hippophaë rhamnoides) as an example food matrix. The targeted strategy combines 1) fatty acid profiling with GC, 2) separation of TAGs with RP-HPLC, 3) stereospecific separation with chiral-phase HPLC and 4) structural characterization with MS. Three major asymmetric diacid- and triacid-TAG species were analyzed in sea buckthorn pulp oil. Off-line coupling of RP-HPLC and chiral-phase HPLC allowed separation of several TAG regioisomers and enantiomers, which could not be resolved using one-dimensional techniques. Enantiomeric ratios were determined and specific structural analysis of separated TAGs was performed using direct inlet ammonia negative ion chemical ionization method. Of the TAG 160/161/161 palmitic acid (C160) was located predominantly in a primary position and the enantiomeric ratio of TAG sn-161-161-160 to sn-160-161-161 was 70.5/29.5. Among the TAGs 160/160/182 and 160/160/161, only ca 5% had C160 in the sn-2 position, thus, ca 95% were symmetric sn-160-182-160 and sn-160-161-160. The enantiomeric ratio of triacid-TAGs containing C160 and two unsaturated fatty acids (palmitoleic C161, oleic C181 or linoleic acids C182) could not be resolved due to lack of commercial enantiopure reference compounds. However, it became clear that the targeted strategy presented offer unique and convenient method to study the enantiomeric structure of individual TAGs.In the development of biocompatible materials for biomedical applications, infections and their resulting inflammation responses are important issues caused typically by the adhesion of micro-organisms on medical devices. Recently slippery liquid-infused porous surfaces (SLIPS) has provided a new strategy for anti-biofouling and low-adhesion surfaces, however, there are still some bottlenecks in practical uses, particularly the loss of lubricant significantly restricts the durability and stability of SLIPS. In this paper, we micro-fabricated well-controlled micro-cavities with different profiles (vertical or inclined walls) to investigate the long-term anti-biofouling effect of SLIPS. We explored microstructure geometries in two aspects the aspect ratio and the slope angle relevant with the Laplace pressure and the oil contact area which lead to different oil-locking abilities. High aspect ratio and inclined slope were demonstrated with better oil-locking ability as well as significantly increased anti-fouling performances. Under the same experimental setup, the Escherichia coli and Staphylococcus aureus bacteria coverage on SLIPS with 80 μm-depth 20° inclined micro-cavities was only ∼30 % of that with vertical micro-cavities, while increasing aspect ratio by 4 times induced ∼3 times enhanced anti-fouling effect. On basis of these findings, we propose the enhanced SLIPS with inclined microstructures to achieve better oil-locking ability and long-term anti-biofouling performance, which may broaden many practical applications of SLIPS.