Hepatocellular Carcinoma (HCC) patients usually have a high rate of relapse and metastasis. Alcohol, a risk factor for HCC, promotes the aggressiveness of HCC. However, the basic mechanism is still unclear. We used HCC cells and an orthotopic liver tumor model of HCC-LM3 cells for BALB/C nude mice to study the mechanism of alcohol-induced HCC progression. We showed that chronic alcohol exposure promoted HCC cells metastasis and pulmonary nodules formation. First, we identified miR-22-3p as an oncogene in HCC, which promoted HCC cells stemness, tumor growth, and metastasis. Further, we found that miR-22-3p directly targeted TET2 in HCC. TET2, a dioxygenase involved in cytosine demethylation, has pleiotropic roles in hematopoietic stem cells self-renewal. In clinic HCC specimen, TET2 expression was not only decreased by alcohol consumption, but also inversely correlated with miR-22-3p levels. Then, we demonstrated that TET2 depletion promoted HCC cells stemness, tumor growth and metastasis. Furthermore, we identified that β-catenin was an upstream activator of miR-22-3p. In conclusion, this study suggests that chronic alcohol exposure promotes HCC progression and β-catenin/miR-22-3p/TET2 regulatory axis plays an important role in alcohol-promoted HCC malignancy.Until recently, little was known about the mechanism for killing and clearing bacteria from the bloodstream. Leukocyte phagocytosis could not be a mechanism for catching, killing and removing bacteria from the bloodstream because of many reasons. Recently accumulated data have led to the conclusion that in bacteremia, bacteria are quickly removed from the blood and erythrocytes are the main cells that capture, kill and remove bacteria.  https://www.selleckchem.com/products/azd0156-azd-0156.html  Data were also obtained that erythrocytes catch bacteria by triboelectric charge attraction and kill them by oxygen released from oxyhemoglobin. This phenomenon has been named oxycytosis by analogy with the term phagocytosis. Oxycytosis has been discussed in a number of published articles, but the specific mechanism of triboelectric charging and the mechanism of killing bacteria by oxidation, have not yet been detailed. The purpose of this review is to provide a more detailed explanation of the process of triboelectric charging and capture of bacteria by erythrocytes and destruction of bacteria by oxidation. For the first time, the review presents various variants of oxycytosis (two-stage, three-stage, multi-stage), depending on the resistance of the pathogen to oxidation. The review also discusses the biological significance of oxycytosis and its impact on the understanding of bacteremia and sepsis.
  During the COVID-19 lockdown, problematic Internet use (PIU) has become a serious issue among residential college students, who remain physically isolated from off-campus society. This study constructs an integrated model to investigate the influencing mechanisms of internal locus of control (LOC) and objective peer effects.
 
  Residential college students (n = 494) were surveyed from a single department of a Chinese university. An item from the World Value Survey was employed to measure internal LOC, while objective peer effects were assessed via friends' mutual nominations. Finally, PIU was measured using Young's Internet Addiction Tests, while a social network analysis and logit regression were combined to estimate various factors' effects on PIU.
 
  In our sample, the prevalence rate of PIU was 30.6%, and while internal LOC was a protective factor for PIU, its protective role was diluted when exposed to a peer environment with high PIU prevalence. Furthermore, indegree performed contrasting roles on PIU under various network conditions. It acted as a protective factor when exposed to a low prevalence of PIU in a peer environment; however, it became a risk factor when PIU peers were prevalent. Lastly, the protective efficacy of betweenness was activated when individuals had more than one PIU friend.
 
  Further intervention studies focusing on individuals with a weak internal LOC are recommended during the lockdown. Additionally, interventions that consider the network structures carefully, may enhance the prevention of PIU.
 Further intervention studies focusing on individuals with a weak internal LOC are recommended during the lockdown. Additionally, interventions that consider the network structures carefully, may enhance the prevention of PIU.
  Despite cardiovascular disease (CVD) risk prediction equations becoming more widely available for people aged 75 years and over, views of older people on CVD risk assessment are unknown.
 
  To explore older people's views on CVD risk prediction and its assessment.
 
  Qualitative study of community dwelling older New Zealanders.
 
  We purposively recruited a diverse group of older people. Semi-structured interviews and focus groups were conducted, transcribed verbatim and thematically analysed.
 
  Thirty-nine participants (mean age 74 years) of Māori, Pacific, South Asian and European ethnicities participated in one of 26 interviews or three focus groups. Three key themes emerged, (1) Poor knowledge and understanding of cardiovascular disease and its risk assessment, (2) Acceptability and perceived benefit of knowing and receiving advice on managing personal cardiovascular risk; and (3) Distinguishing between CVD outcomes; stroke and heart attack are not the same. Most participants did not understand CVD terms but were familiar with 'heart attack,' 'stroke' and understood lifestyle risk factors for these events. Participants valued CVD outcomes differently, fearing stroke and disability which might adversely affect independence and quality of life, but being less concerned about a heart attack, perceived as causing less disability and swifter death. These findings and preferences were similar across ethnic groups.
 
  Older people want to know their CVD risk and how to manage it, but distinguish between CVD outcomes. To inform clinical decision making for older people, risk prediction tools should provide separate event types rather than just composite outcomes.
 Older people want to know their CVD risk and how to manage it, but distinguish between CVD outcomes. To inform clinical decision making for older people, risk prediction tools should provide separate event types rather than just composite outcomes.