Compounds 10 (6,7-dimethoxy-2,3,4,11b-tetrahydro-1H-fluoreno[9,1-cd]azepine) and 12 (6,7-dimethoxy-2-methyl-2,3,4,11b-tetrahydro-1H-fluoreno[9,1-cd]azepine) have been identified as structurally novel, high affinity (Ki  = 5 nM), selective 5-HT6 receptor ligands. © 2020 John Wiley & Sons A/S.In the current issue of BJOG, Andolf and colleagues use a national register to investigate the association between placental bed disorders and later dementia (BJOG 2020 xxxx). The authors consider a number of placental bed disorders in relation to both vascular and non-vascular dementia, as well as Alzheimer's, and identify an increased risk of vascular dementia in women with previous pregnancy-induced hypertension, preeclampsia, spontaneous preterm labor and birth or preterm premature rupture of membranes. This article is protected by copyright. All rights reserved.In response to the energy demand triggered by developmental signals and environmental stressors, the cells launch the mitochondrial biogenesis process. This is a self-renewal route, by which new mitochondria are generated from the ones already existing. Recently, considerable progress has been made in deciphering mitochondrial biogenesis-related proteins and genes that function in health and in pathology-related circumstances. However, an outlook on the intracellular mechanisms shared by the main players that drive mitochondrial biogenesis machinery is still missing. Here, we provide such a view by focusing on the following issues (a) the role of mitochondrial biogenesis in homeostasis of the mitochondrial mass and function, (b) the signalling pathways beyond the induction/promotion, stimulation and inhibition of mitochondrial biogenesis and (c) the therapeutic applications aiming the repair and regeneration of defective mitochondrial biogenesis (in ageing, metabolic diseases, neurodegeneration and cancer). The review is concluded by the perspectives of mitochondrial medicine and research. © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.BACKGROUND Rapid spread of the SARS-CoV-2 virus and concern for viral transmission by ambulatory patients with minimal to no symptoms underline the importance of identifying early or subclinical symptoms of Covid-19 infection. Two such candidate symptoms include anecdotally reported loss of smell and taste. Understanding the timing and association of smell/taste loss in Covid-19 may help facilitate screening and early isolation of cases. METHODS A single-institution, cross-sectional study evaluating patient-reported symptoms with a focus on smell and taste was conducted using an internet-based platform on adult subjects who underwent testing for Covid-19. Logistic regression was employed to identify symptoms associated with Covid-19 positivity. RESULTS A total of 1480 patients with influenza-like symptoms underwent Covid-19 testing between March 3 through 29, 2020. Our study captured 59 of 102 (58%) Covid-19-positive patients and 203 of 1378 (15%) Covid-19-negative patients. Smell and taste loss were reported in 68% (40/59) and 71% (42/59) of Covid-19-positive subjects, respectively, compared to 16% (33/203) and 17% (35/203) of Covid-19-negative patients (p less then 0.001). Smell and taste impairment were independently and strongly associated with Covid-19-positivity (anosmia adjusted odds ratio [aOR] 10.9, 95%CI5.08-23.5; ageusia aOR 10.2 95%CI4.74-22.1); whereas, sore throat was associated with Covid-19-negativity (aOR 0.23, 95%CI0.11-0.50). Of patients who reported Covid-19-associated loss of smell, 74% (28/38) reported resolution of anosmia with clinical resolution of illness. CONCLUSIONS In ambulatory individuals with influenza-like symptoms, chemosensory dysfunction was strongly associated with Covid-19 infection and should be considered when screening symptoms. Most will recover chemosensory function within weeks paralleling resolution of other disease-related symptoms. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.Virtual flow diverter deployment techniques underwent significant development during the last couple of years. Each existing technique displays advantageous features, as well as significant limitations.  https://www.selleckchem.com/  One common drawback is the lack of quantitative validation of the mechanics of the device. In the following work, we present a new spring-mass-based method with validated mechanical responses that combines many of the useful capabilities of previous techniques. The structure of the virtual braids naturally incorporates the axial length changes as a function of the local expansion diameter. The force response of the model was calibrated using the measured response of real FDs. The mechanics of the model allows to replicate the expansion process during deployment, including additional effects such as the push-pull technique that is required for the deployment of braided FDs to achieve full opening and proper wall apposition. Furthermore, it is a computationally highly efficient solution that requires little pre-processing and has a run-time of a few seconds on a general laptop and thus allows for exploratory analyses. The model was applied in a patient-specific geometry, where corresponding accurate control measurements in a 3D-printed model were also available. The analysis shows the effects of FD oversizing and push-pull application on the radial expansion, surface density, and on the wall contact pressure. © 2020 The Authors. International Journal for Numerical Methods in Biomedical Engineering published by John Wiley & Sons Ltd.Studies focusing on marine macrophyte metabarcoding from environmental samples are scarce, due to the lack of a universal barcode for these taxa, and to their poor representation in DNA databases. Here, we searched for a short barcode able to identify marine macrophytes from tissue samples; then, we created a DNA reference library which was used to identify macrophytes in eDNA from coastal sediments. Barcoding of seagrasses, mangroves and marine macroalgae (Chlorophyta, Rhodophyta and Phaeophyceae) was tested using 18 primer pairs from six barcoding genes the plant barcodes rbcL, matK and trnL, plus the genes ITS2, COI and 18S. The 18S gene showed the highest universality among marine macrophytes, amplifying 95-100% of samples; amplification performance of the other barcodes was limited. Taxonomy was assigned using a phylogeny-based approach to create an 18S DNA reference library. Macrophyte tissue sequences were accurately identified within their phyla (88%), order (76%), genus (71%) and species (23%). Nevertheless, out of 86 macrophytes tested, only 48% and 15% had a reference sequence at genus and at species level, respectively.