https://www.selleckchem.com/products/tvb-2640.html The objective of this study was to investigate the effects of dietary supplementation with different n-6/n-3 polyunsaturated fatty acid (PUFA) ratios on growth performance, meat quality, and fatty acid profile in Heigai pigs. A total of 54 Heigai finishing pigs (body weight 71.59 ± 2.16 kg) were randomly divided into three treatments with six replications (three pigs per replication) and fed diets containing different n-6/n-3 PUFA ratios 81, 51, and 31. Pigs fed the dietary n-6/n-3 PUFA ratio of 81 had the highest feed to gain ratio (p less then 0.01), carcass weight (p less then 0.05), redness a* (p less then 0.01), and yellowness b* (p less then 0.01). Fatty acid compositions in longissimus dorsi muscle (LDM) and subcutaneous adipose tissue (SAT) were significantly changed (p less then 0.01). Notably, the meat from the pigs fed with the low dietary n-6/n-3 PUFA ratio had higher n-3 PUFA contents (p less then 0.01) and lower n-6/n-3 PUFA ratio (p less then 0.01). The triglyceride and total cholesterol contents were significantly decreased in SAT from the pigs fed with dietary n-6/n-3 PUFA ratios of 51 (p less then 0.05) and 31 (p less then 0.01). Reducing n-6/n-3 PUFA ratio upregulated the expression of HSL (p less then 0.05), CPT1 (p less then 0.01), and FABP4 (p less then 0.01) but downregulated ATGL (p less then 0.01) expression. These results demonstrate that the lower n-6/n-3 PUFA ratio regulates meat quality and enhances the deposition of n-3 PUFA in Heigai pigs.Sardinia, an island located to the west of Italy in the Mediterranean Sea, boasts three native horse breeds Giara, Sarcidano, and Sardinian Anglo-Arab. Here, we have investigated for the first time three loci of the non-recombining region of the Y chromosome (NRY) in 34 stallions from these breeds and performed a phylogenetic analysis of the maternal relationships among 178 previously published mitochondrial control regions. We found that the current