Water splitting for production of hydrogen as a clean energy alternative to fossil fuel has received much attention, but it is still a tough challenge to synthesize electrocatalysts with controllable bonding and charge distribution. In this work, ultrafine S-doped RuP nanoparticles homogeneously embedded in a N, P, and S-codoped carbon sheet (S-RuP@NPSC) is synthesized by pyrolysis of poly(cyclotriphosphazene-co-4,4'-sulfonyldiphenol) (PZS) as the source of C/N/S/P. The bondings between Ru and N, P, S in PZS are regulated to synthesize RuS2 (800 °C) and S-RuP (900 °C) by different calcination temperatures. The S-RuP@NPSC with low Ru loading of 0.8 wt% with abundant active catalytic sites possesses high utilization of Ru, the mass catalytic activity is 22.88 times than 20 wt% Pt/C with the overpotential of 250 mV. Density functional theory calculation confirms that the surface Ru (-0.18 eV) and P (0.05 eV) are catalytic active sites for the hydrogen evolution reaction (HER), and the according charge redistribution of Ru is regulated by S and P with reverse electronegativity and electron-donor property to induce a synergistically enhanced reactivity toward the HER. This work provides a rational method to regulate the bonding and charge distribution of Ru-based electrocatalysts by reacting macromolecules with multielement of C/N/S/P with Ru.Silicon, with its elaborate microstructure, plays important roles in energy materials. In operando engineering of microstructure during extraction is an ideal protocol to develop advanced Si-based materials. A template-free electrochemical preparation of silicon nanotubes (Si-NT) is herein achieved by co-electrolysis of SiO2 and AgCl in molten NaCl-CaCl2 at 850 °C. The in situ electrodeposited Ag facilitates the generation of a liquid Ag-Si intermediate, triggering a liquid-solid mechanism to direct the growth of Si-NT. An automatic separation of Ag from Si then occurs in the following cooling process, resulting in Ag deposits on the Ni current collector and recycling of Ag. Such a facile and smart preparation of Si-NT from affordable silica guarantees an enhanced current efficiency of 74%, a decreased energy consumption of 12.1 kW h kgSi-1, and enhanced lithium-storage capability of the electrolytic Si-NT. An in situ coating of Ag over the Si-NT can also be fulfilled by simply introducing soluble AgCl in the melts. The present study provides a template-free preparation and an in situ surface modification of Si-NT.Gene editing is a crucial and effective strategy to treat genetic diseases. Safe and effective delivery vectors are specially required for efficient gene editing in vivo of CRISPR/Cas9 system. Interestingly, lactose, a natural saccharide, can specifically bind to asialoglycoprotein receptors, highly expressed on the surface of hepatocellular carcinoma (HCC) cells.  https://www.selleckchem.com/products/ziftomenib.html  Herein, a lactose-derived branched cationic biopolymer (LBP) with plentiful reducible disulfide linkages and hydroxyl groups is proposed as a potential delivery vector of CRISPR/Cas9 system for efficient genome editing in vivo to treat orthotopic HCC. LBP is synthesized via a facile one-pot ring-opening reaction. LBP possesses excellent compacting ability, degradability, biocompatibility, gene transfection performances, and HCC-targeting ability. LBP-mediated delivery of classical pCas9-survivin, which can target and knockout survivin oncogene, produces efficient gene editing performances, and superb anti-cancer activities in orthotopic HCC mouse models. This study provides an attractive and safe strategy for the rational design of CRISPR/Cas9 delivery system.In order to mitigate antibiotic resistance, a new strategy to increase antibiotic potency and reverse drug resistance is needed. Herein, the translocation mechanism of an antimicrobial guanidinium-functionalized polycarbonate is leveraged in combination with traditional antibiotics to afford a potent treatment for drug-resistant bacteria. Particularly, this polymer-antibiotic combination approach reverses rifampicin resistance phenotype in Acinetobacter baumannii demonstrating a 2.5 × 105-fold reduction in minimum inhibitory concentration (MIC) and a 4096-fold reduction in minimum bactericidal concentration (MBC). This approach also enables the repurposing of auranofin as an antibiotic against multidrug-resistant (MDR) Gram-negative bacteria with a 512-fold MIC and 128-fold MBC reduction, respectively. Finally, the in vivo efficacy of polymer-rifampicin combination is demonstrated in a MDR bacteremia mouse model. This combination approach lays foundational ground rules for a new class of antibiotic adjuvants capable of reversing drug resistance phenotype and repurposing drugs against MDR Gram-negative bacteria.Human pluripotent stem cells (hPSCs) are a potent source of clinically relevant mesenchymal stem cells (MSCs) that confer functional and structural benefits in cell therapy and tissue regeneration. Obtaining sufficient numbers of MSCs in a short period of time and enhancing the differentiation potential of MSCs can be offered the potential to improve the regenerative activity of MSCs therapy. In addition, the underlying processes in the isolation and derivation of MSCs from hPSCs are still poorly understood and controlled. To overcome these clinical needs, an efficient and simplified technique on the isolation of MSCs from spontaneously differentiated human embryonic stem cells (hESCs) via integrin α5β1 (fibronectin (FN) receptor)-to-FN interactions (hESC-FN-MSCs) is successfully developed. It is demonstrated that hESC-FN-MSCs exhibit a typical MSC surface phenotype, cellular morphology, with the whole transcriptome similar to conventional adult MSCs; but show higher proliferative capacity, more efficient trilineage differentiation, enhanced cytokine secretion, and attenuated cellular senescence. In addition, the therapeutic potential and regenerative capacity of the isolated hESC-FN-MSCs are confirmed by in vitro and in vivo multilineage differentiation. This novel method will be useful in the generation of abundant amounts of clinically relevant MSCs for stem cell therapeutics and regenerative medicine.