Biosolids were applied as a fertilizer after drying, torrefaction (220, 320 °C), and pyrolysis (420, 520, 620 °C). Lettuce was grown on contrasting soils, and the transfer of pharmaceuticals to aboveground biomass was assessed. Of 42 compounds detected in dried biosolids, 10 were found in lettuce. Their potency for translocation to aerial parts was in the order ethenzamide > carbamazepine > mirtazapine~tramadol > N-desmethyltramadol~solifenacin > sertraline~trazodone~venlafaxine > propafenone. Application of dried biosolids resulted in the highest uptake of pharmaceuticals and the neutral soil further intensified the uptake due to prevalent neutral speciation of the ionizable basic molecules. Torrefaction reduced the total pharmaceutical content in biosolids by 92.2% and 99.5% at 220 and 320 °C, respectively. Torrefied biosolids significantly reduced the uptake of pharmaceuticals and led to the highest biomass on acidic soil but were phytotoxic on the neutral soil. Pyrolysed biosolids increased the biomass production of lettuce on both soils and blocked the uptake of pharmaceuticals. A minimum biosolids pyrolysis temperature of 420 °C should be ensured prior to soil application as it represents a good compromise between fertilization potential, pharmaceutical uptake, and homogeneity of plant response regardless of the soil characteristics.Accumulation of arsenite [As(III)] and dimethylarsinic acid (DMA) in rice grainsposes a threat to human health. Although silicon (Si) has been reported to reduce As uptake, the mechanisms involved are not fully understood. In this study, we first confirmed that the concurrent addition of Si and As in solution decreased As accumulation in rice. Then, the effect of Si previously deposited in shoots by the pretreatment of rice seedlings with Si for one week was investigated by using lsi2 mutant and its wild type. The uptake of both As(III) and DMA decreased in rice subjected concurrently to Si and As (III)/DMA in solution, without effects on OsLsi1 and OsLsi2 expression. This concurrent treatment also decreased total As concentration in the root cell walls and xylem sap, which might have restrained apoplastic transport of As to shoots. Silicon previously deposited in the shoots decreased root-to-shoot As(III) translocation and down-regulated OsLsi1, OsLsi2 and OsNRAMP1, but did not affect As concentration in the roots, and had no effect on DMA uptake and accumulation in shoots and roots either. This study sheds light on the role of silicon in solution and rice shoots in As(Ⅲ) and DMA uptake and transport by rice.Sludge-derived biochar (SDBC) has been regarded as persulfate (PS) activator during the remediation of organic contamination. However, the complexity of sludge composition makes it difficult to predict the activity of SDBC and the efficacy of PS. To improve the understanding of how the composition of sludge regulated activity of its parent SDBC towards PS activation, we used two SDBCs derived from different sludges with significantly different organic compositions and metals. Results indicated the higher content of organic and nitrogen content in sludge led to higher polymerization and condensation of carbon layer and more moieties in SDBC1, whereas more Fe species (e.g. Fe-O, Fe2+ and Fe3+) formed in SDBC2. According to the results of phenol (PN) degradation in SDBC/PS, the apparent rate constants (kobs) of SDBC2-700 (0.0037 min-1) was 2 folds higher than that of SDBC1-700 (0.0016 min-1), whereas the SDBC1-500 (6.0 ×10-4 min-1) exhibited higher kobs than that of SDBC2-500 (4.9 ×10-4 min-1). The difference of PS activation by different SDBCs mainly relied on generated reactive oxygen species (ROS). The persistent free radicals (PFRs) and Fe species acted as redox sites for generated ROS, which were depended on the organic compositions and involved metals in used sludges.
  Immune checkpoint blockade (ICB) therapy shows limited efficacy in ovarian cancers due to the "cold" immune phenotype surrounding these tumors. Previous studies have shown that in ovarian cancer Wnt/β-catenin pathway activation contributes to this immune phenotype. Here, we evaluated the anti-tumor and immune-enhancing properties of the Wnt inhibitor, CGX-1321, used alone or in combination with either DKN-01 or anti-PD-1 therapy, in pre-clinical ovarian cancer models.
 
  The parental ID8 murine ovarian cancer model harboring a knock-out of p53 (ID8p53
  ) and MISIIR-Tag spontaneous ovarian cancer models were used to test the effects of CGX-1321 alone or in combination therapies on tumor burden and immune cell landscape in the tumor microenvironment (TME). Flow cytometry and NanoString analyses were used to characterize the changes in tumor-intrinsic signaling and immune-related profiles in the TME of ovarian cancer in response to treatments.
 
  CGX-1321 significantly reduced tumor burden and constrained tumor progression in the ID8p53
  and MISIIR-Tag models. Furthermore, CGX-1321 increased infiltrating CD8+ T cells in the TME. Combining CGX-1321 with either DKN-01 or anti-PD-1 therapy also decreased tumor burden and increased CD8+ T cell infiltration in the omentum TME but did not do so to a greater extent that CGX-1321 monotherapy.
 
  CGX-1321 significantly reduced tumor burden and enhanced CD8+ T cell levels in ovarian cancer, nevertheless the addition of DKN-01 or anti-PD-1 therapies did not enhance these effects of CGX-1321. Further investigation is needed to determine if CGX-1321+DKN-01 combination treatment sensitizes pre-clinical ovarian cancer to ICB therapy.
 CGX-1321 significantly reduced tumor burden and enhanced CD8+ T cell levels in ovarian cancer, nevertheless the addition of DKN-01 or anti-PD-1 therapies did not enhance these effects of CGX-1321. Further investigation is needed to determine if CGX-1321 + DKN-01 combination treatment sensitizes pre-clinical ovarian cancer to ICB therapy.
  Cytomegalovirus (CMV) is a common infection that establishes latency in healthy people. CMV has been associated with alterations of the immune compartment leading to improved responses, while inflammation has been shown to adversely impact outcomes. We investigated whether CMV serostatus predicts outcomes in ovarian cancer in the presence or absence of inflammation.
 
  A total of 106 patients with serous ovarian cancer from 2006 to 2009 were analyzed.  https://www.selleckchem.com/products/ver155008.html  CMV and systemic inflammation was measured using CMV immunoglobulin G (IgG) and C-reactive protein (CRP), respectively, in serum collected prior to cytoreduction. Patients were stratified by CMV IgG (non-reactive, reactive/borderline) and CRP (≤10, >10mg/L) status. Overall survival (OS) and recurrence-free survival (RFS) were compared by group using log-rank tests and Cox proportional hazards regression models adjusting for age at surgery.
 
  Of 106 eligible patients, 40 (37.7%) were CMV+/CRP+, 24 (22.6%) CMV+/CRP-, 19 (17.9%) CMV-/CRP+, and 23 (21.7%) CMV-/CRP-.