ation in BCNC rat model.This study aimed to carry out a systematic review of meta-analysis and risk assessment of the global pooled concentration of fluoride and identify the influencing factors of fluoride loads in groundwater. The study extracted data from 36 most prevalent regions of 20 countries in the world through various search engines using keywords as well as Boolean operators and follows the PRISMA statement in every phase of literature searching. The study illustrated the pooled concentration of fluoride in the selected 20 countries was 2.1267 mg/L (1.650, 2.604) at 95% confidence interval, which was higher than the WHO standards limit of 1.5 mg/L.  https://www.selleckchem.com/CDK.html  The results of the meta-analysis suggested that pH, Na, HCO3 and rainfall was significantly positively correlated (r = ~0.4; p ˂ 0.05) to fluoride and has a weak correction with the other parameters such as local temperature, altitude, water depth, EC, Ca, and Mg (r = -0.064 to +0.214; p ˂ 0.05). Concerning, the risk assessment through oral and dermal route exposure revealed that the consumers in most of the regions were at considerable risk, and the children were more vulnerable than the adults (THQ > 1). The study findings would help to take measures for safe water supply in the affected areas. PRACTITIONER POINTS The calculated pooled concentration of fluoride was 2.1267 mg/L (1.650, 2.604) at 95% CI in groundwater of the study areas. Climatic conditions and lithological composition are the key controlling factors for groundwater fluoride contamination. Semi-arid and arid regions are significantly affected by fluoride rather than humid areas. Regarding fluorosis in selected regions, children (78%) are more vulnerable than adults (23%).Liver transplantation is currently the only curative treatment for several liver diseases such as acute liver failure, end-stage liver disorders, primary liver cancers and certain genetic conditions. Unfortunately, despite improvements to transplantation techniques, including live donor transplantation, the number of organs available remains insufficient to meet patient needs. Hepatocyte transplantation has enabled some encouraging results as an alternative to organ transplantation, but primary hepatocytes are little available and cannot be amplified using traditional 2D culture systems. Indeed, although recent studies have tended to show that 3D culture enables long term hepatocyte culture, it is still agreed that like most adult primary cell types, they remain refractory to in vitro expansion. Because of their exceptional properties, human pluripotent stem cells (hPSCs) can be amplified indefinitely and differentiated into any cell type, including liver cells. While many teams have worked on hepatocyte differentiation, there has been a consensus that cells obtained after hPSC differentiation have more foetal than adult hepatocyte characteristics. New technologies have been used to improve the differentiation process in recent years. This review discusses the technical improvements made to hepatocyte differentiation protocols and the clinical approaches developed to date and anticipated in the near future.
  To investigate prion protein (PrP) deposits in cutaneous tissues of patients of glycosylphosphatidylinositol (GPI)-anchorless prion diseases with neuropathy.
 
  Cutaneous tissue samples from three patients with GPI-anchorless prion diseases were obtained, two cutaneous biopsy samples from the lower leg of Case 1 (Y162X) and Case 3 (D178fs25), and a cutaneous sample taken from the abdomen during an autopsy of Case 2 (D178fs25). We performed immunohistochemistry for PrP to look for abnormal PrP deposits.
 
  PrP deposits were observed in the dermal papilla, the sweat glands, the hair follicles, the arrector pili muscles, and peripheral nerves of all examined cases of GPI-anchorless prion disease with neuropathy. The abnormal PrP accumulation was frequently localized at the basement membrane, and colocalized with laminin.
 
  Immunohistochemical detection of PrP in cutaneous samples could be used to definitively diagnose GPI-anchorless PrP disease with neuropathy.
 Immunohistochemical detection of PrP in cutaneous samples could be used to definitively diagnose GPI-anchorless PrP disease with neuropathy.Thailand has committed to reducing population sodium intake by 30% by 2025. However, reliable nationally representative data are unavailable for monitoring progress toward the goal. We estimated dietary sodium consumption using 24-hour urinary analyses in a nationally representative, cross-sectional population-based survey. We selected 2388 adults (aged ≥ 18 years) from the North, South, North-east, Central Regions, and Bangkok, using multi-stage cluster sampling. Mean sodium excretion was inflated by 10% to adjust for non-urinary sources. Multivariate logistic regression was performed to assess factors associated with sodium consumption ≥ 2000 mg. Among 1599 (67%) who completed urine collection, mean age was 43 years, 53% were female, and 30% had hypertension. Mean dietary sodium intake (mg/day) was 3636 (±1722), highest in South (4108 ± 1677), and lowest in North-east (3316 ± 1608). Higher sodium consumption was independently associated with younger age (Adjusted Odds Ratio (AOR) 2.81; 95% Confidence interval (CI) 1.53-5.17; p = .001); higher education (AOR 1.79; 95% CI 1.19-2.67; p = .005), BMI ≥ 25 (AOR 1.55; 95% CI 1.09-2.21; p=.016), and hypertension (AOR 1.58; 95% CI 1.02-2.44; p = .038). Urine potassium excretion was 1221 mg/day with little variation across Regions. Estimated dietary sodium consumption in Thai adults is nearly twice as high as recommended levels. These data provide a benchmark for future monitoring.
  Unequal access to required health care services could deteriorate inequality in health outcomes and increase mortality and morbidity, especially among disadvantaged groups. One of the most debated factors in shaping health care inequalities is gender. This study aimed to measure and explains gendered socioeconomic-related inequality in outpatient health care utilization in Iran.
 
  This is a cross-sectional research conducted at a national level in Iran. The required data was obtained from an Iranian health care utilization household survey conducted in 2015. Erreygers Concentration Index (ECI) was used to measure the socioeconomic inequality in outpatient health care utilization. A regression-based decomposition analysis was also used to explain socioeconomic-related inequalities.
 
  There was a significant pro-rich inequality in outpatient health care utilization between males (ECI=0.115, SE=0.014) and females (ECI=0.083 SE=0.011) indicating that utilization of these services was concentrated on the better-offs.