We further isolated four bacterial strains from fly guts and the supplementation of individual Lactobacillus plantarum or Acetobacter pomorum strain exacerbated the neuronal and behavioral dysfunction of PD flies, which could not be rescued by EGCG. Transcriptomic analysis identified TotM as the central gene responding to EGCG or microbial manipulations. Genetic ablation of TotM blocked the recovery activity of EGCG, suggesting that EGCG-mediated protection warrants TotM. Apart from familial form, EGCG was also potent in improving sporadic PD symptoms induced by rotenone treatment, wherein gut microbiota shared regulatory roles. Together, our results suggest the relevance of the gut microbiota-TotM pathway in EGCG-mediated neuroprotection, providing insight into indirect mechanisms underlying nutritional intervention of Parkinson's disease. © 2020 The Authors. The FASEB Journal published by Wiley Periodicals, Inc. on behalf of Federation of American Societies for Experimental Biology.BACKGROUND Skin cancer is a well-recognized public health issue, and primary prevention is the most effective strategy for reducing skin cancer risk. The current recommendations are that behavioral counseling for sun safety measures is most beneficial and effective for children and adolescents and that targeting this population at primary and middle schools is the ideal intervention strategy to increase sun-protective behaviors and reduce UV exposure, sunburn incidence, and formation of new moles. Numerous studies on the effectiveness of school-based sun safety interventions among elementary and middle school students have shown an increase in sun safety knowledge, attitudes, and behaviors following the intervention. OBJECTIVE To conduct a pilot feasibility study of "Live Sun Smart!," (LSS) a school-based, multicomponent, interactive sun safety presentation, at changing sun safety knowledge, attitudes, and behaviors among middle school students. METHODS A non-randomized, single-group pretest-posttest interventional pilot study of the LSS program among children enrolled in grade 6. RESULTS After exposure to LSS, participants were more likely to give correct answers to knowledge-based sun safety questions and to report negative attitudes toward tanning. Minimal and not significant changes were found in self-reported sun safety behaviors, though students did report an intention to change behaviors following the intervention. Participants were satisfied with the program and believed it increased their sun safety knowledge. CONCLUSION Live Sun Smart! appears to be an effective school-based, multicomponent sun safety program for improving sun safety knowledge and attitudes toward tanning among middle school students in this initial test of it. The strengths and weaknesses of this pilot study have implications for future research. © 2020 Wiley Periodicals, Inc.The study of sexual dimorphism in dog anatomy, especially with regard to skeletal elements, has received little attention.  https://www.selleckchem.com/TGF-beta.html  The present work focuses on elements of the canine stylo- and zeugopodium, less documented than the skull or pelvis in the literature. In order to identify only sex-dependent effects, we analysed a single breed the German Shepherd Dog. Data come from 25 dogs, with a balanced sex ratio (12 males and 13 females). Four skeletal elements of the forelimb and hindlimb (humerus, radius, femur, tibia) were each measured using seven linear morphometric variables. Univariate and multivariate analyses were then performed on these 28 variables. For all measurements, males are on average larger than females, with a mean sexual dimorphism ratio of 1.07. Sexual dimorphism is significant for 92.8% of the variables. Except of femoral measurements, diaphyseal values show the highest grade of sexual dimorphism. The mean level of disparity is higher in the forelimb (1.08) than in the hindlimb (1.05). A significant dimorphism is shown for the first component of principal component analyses conducted on each skeletal element, and for the second component with humerus measurements. Discriminant functions for sex identification give success rates included between 82% for the radius and 93% for the femur, the latter providing the highest reported score for sex identification in dogs from any skeletal element. These complementary statistic methods highlight a more dimorphic forelimb in size and a more dimorphic hindlimb in shape. © 2020 Blackwell Verlag GmbH.HIV-1 persists in a latent form during antiretroviral therapy, mainly in CD4+ T cells, thus hampering efforts for a cure. HIV-1 infection is accompanied by metabolic alterations, such as oxidative stress, but the effect of cellular antioxidant responses on viral replication and latency is unknown. Here, we show that cells survive retroviral replication, both in vitro and in vivo in SIVmac-infected macaques, by upregulating antioxidant pathways and the intertwined iron import pathway. These changes are associated with remodeling of promyelocytic leukemia protein nuclear bodies (PML NBs), an important constituent of nuclear architecture and a marker of HIV-1 latency. We found that PML NBs are hyper-SUMOylated and that PML protein is degraded via the ubiquitin-proteasome pathway in productively infected cells, before latency establishment and after reactivation. Conversely, normal numbers of PML NBs were restored upon transition to latency or by decreasing oxidative stress or iron content. Our results highlight antioxidant and iron import pathways as determinants of HIV-1 latency and support their pharmacologic inhibition as tools to regulate PML stability and impair latency establishment. © 2020 The Authors. Published under the terms of the CC BY 4.0 license.Eukaryotic origins are inextricably linked with the arrival of a pre-mitochondrion of alphaproteobacterial-like ancestry. However, the nature of the "host" cell and the mode of entry are subject to heavy debate. It is becoming clear that the mutual adaptation of a relatively simple, archaeal host and the endosymbiont has been the defining influence at the beginning of the eukaryotic lineage; however, many still resist such symbiogenic models. In part 1, it is posited that a symbiotic stage before uptake ("pre-symbiosis") seems essential to allow further metabolic integration of the two partners ending in endosymbiosis. Thus, the author argued against phagocytic mechanisms (in which the bacterium is prey or parasite) as the mode of entry. Such positions are still broadly unpopular. Here it is explained why. Evolutionary thinking, especially in the case of eukaryogenesis, is still dominated by anachronistic reasoning, in which highly derived protozoan organisms are seen as in some way representative of intermediate steps during eukaryotic evolution, hence poisoning the debate.