Moreover, it is anticipated that the methodology employed to create this library of new natural-based products could be adapted to modify other polysaccharides and biopolymers in general.Pseudaminic acid (Pse), a unique carbohydrate in surface-associated glycans of pathogenic bacteria, has pivotal roles in virulence. Owing to its significant antigenicity and absence in mammals, Pse is considered an attractive target for vaccination or antibody-based therapies against bacterial infections. However, a specific and universal probe for Pse, which could also be used in immunotherapy, has not been reported. In a prior study, we used a tail spike protein from a bacteriophage (ΦAB6TSP) that digests Pse-containing exopolysaccharide (EPS) from Acinetobacter baumannii strain 54149 (Ab-54149) to form a glycoconjugate for preparing anti-Ab-54149 EPS serum. We report here that a catalytically inactive ΦAB6TSP (I-ΦAB6TSP) retains binding ability toward Pse. In addition, an I-ΦAB6TSP-DyLight-650 conjugate (Dy-I-ΦAB6TSP) was more sensitive in detecting Ab-54149 than an antibody purified from anti- Ab-54149 EPS serum. Dy-I-ΦAB6TSP also cross-reacted with other pathogenic bacteria containing Pse on their surface polysaccharides (e.g., Helicobacter pylori and Enterobacter cloacae), revealing it to be a promising probe for detecting Pse across bacterial species. We also developed a detection method that employs I-ΦAB6TSP immobilized on microtiter plate. These results suggested that the anti-Ab-54149 EPS serum would exhibit cross-reactivity to Pse on other organisms. When this was tested, this serum facilitated complement-mediated killing of H. pylori and E. cloacae, indicating its potential as a cross-species antibacterial agent. This work opens new avenues for diagnosis and treatment of multidrug resistant (MDR) bacterial infections.The isoreticular principle has been applied to construct two copper metal-organic framework (MOF) analogues with different porosities for the adsorptive capture of CO2 from N2 and CH4 at 1 atm and 298 K. By using a 4-substituted isophthalate linker with a bulky nitro group, the microporous MOF [Cu(BDC-NO2)(DMF)] (UTSA-93 or CuBDC-NO 2 ; H2BDC-NO2 = 4-nitroisophthalic acid and DMF = N,N'-dimethylformamide) has been synthesized with mot topology, showing a compact pore structure with a size of 6.0 × 7.0 Å2 in contrast to that of 6.9 × 8.5 Å2 in the prototypical MOF with a bromo group. The optimized pore structure allows the nitro-functionalized MOF to capture CO2 with a higher capacity of about 2.40 mmol g-1 under ambient conditions, in contrast to 1.08 mmol g-1 in the bromo-functionalized analogue. The adsorption selectivity of CuBDC-NO 2 -a for a CO2/N2 (1585) mixture (28) under ambient conditions is also higher than that of the bromo-substituted prototype (25) and comparable with those of several MOF materials. Moreover, dynamic breakthrough experiments of the nitro-functionalized MOF have been performed to illustrate its separation potential toward a CO2/N2 mixture.Herein, high-performance, reliable electrochromic supercapacitors (ECSs) are proposed based on tungsten trioxide (WO3) and nickel oxide (NiO) films. To maximize device performance and stability, the stoichiometric balance between anode and cathode materials is controlled by carefully adjusting the thickness of the anodic NiO film while fixing the thickness of WO3 to ∼660 nm. Then, a small amount (≤10 mol %) of metal (e.g., copper) is doped into the NiO film, improving the electrical conductivity and electrochemical activity. At a Cu doping level of 7 mol %, the resulting ECS exhibited the highest performance, including a high areal capacitance (∼14.9 mF/cm2), excellent coulombic efficiency (∼99%), wide operating temperature range (0-80 °C), reliable operation with high charging/discharging cyclic stability (>10,000 cycles), and good self-discharging durability. Simultaneously, the change in transmittance of the device is well synchronized with the galvanostatic charging/discharging curve by which the real-time energy storage status is visually indicated. Furthermore, the practical feasibility of the device is successfully demonstrated. These results imply that the ECS fabricated in this work is a promising potential energy storage platform and an attractive component for future electronics.High-throughput glycan analysis has become an important part of biopharmaceutical production and quality control. However, it is still a significant challenge in the field of glycomics to easily deduce isomeric glycan structures, especially in a high-throughput manner. Ion mobility spectrometry (IMS) is an excellent tool for differentiating isomeric glycan structures. However, demonstrations of the utility of IMS in high-throughput workflows such as liquid chromatography-fluorescence-mass spectrometry (LC-FLR-MS) workflows have been limited with only a small amount of collision cross section (CCS) data available. In particular, IMS data of glycan fragments obtained in positive ion mode are limited in comparison to those obtained in negative ion mode despite positive ion mode being widely used for glycomics. Here, we describe IMS TWCCSN2 data obtained from a high-throughput LC-FLR-IMS-MS workflow in positive ion mode. We obtained IMS data from a selection of RapiFluor-MS (RFMS) labeled N-glycans and also glycopeptides. We describe how IMS is able to distinguish isomeric N-glycans and glycopeptides using both intact IMS and fragment-based IMS glycan sequencing experiments in positive ion mode, without significantly altering the high-throughput nature of the analysis.  https://www.selleckchem.com/products/tasquinimod.html  For the first time, we were able to successfully use IMS in positive ion mode to determine the branching of isomeric glycopeptides and RFMS labeled glycans. Further, we highlight that IMS glycan sequencing of fragments obtained from RFMS labeled glycans was similar to that of glycopeptides. Finally, we show that the IMS glycan sequencing approach can highlight shared structural features of nonisomeric glycans in a high-throughput LC-FLR-IMS-MS workflow.A popular practice in Li-S battery research is to utilize highly nanostructured hosts and excessive electrolytes to enhance sulfur-specific capacities. However, from the perspective of commercialization, this is a less meaningful approach in the pursuit of high-energy Li-S batteries. Herein, we report the fabrication of a nanoskin composed of a conjugated microporous polymer by electropolymerization to create a closed system for a sulfur cathode. The nanoskin is ultrathin, conductive, continuous, and contains uniform micropores of approximately 0.8 nm. The nanoskin sealing prevents the shuttling of polysulfide species without using the absorption effect, enhances the utilization of electrolytes, and allows a fast transport of lithium ions. As a result, the Li-S batteries comprising the cathode with nanoskin exhibit superior stability (∼86% capacity retention) under lean electrolyte conditions and a prolonged lifetime (1000 cycles). At a low electrolyte/sulfur ratio of 4 μL mg-1, the designed cathode delivered a practical energy density of over 300 Wh kg-1 without using any sophisticated hosts.