Use of different interpretation criteria for CSF AD biomarkers produces diverse results. AD pathology is common in patients fulfilling "probable" CBD criteria. CBD diagnostic criteria may have suboptimal positive predictive value. A consensus regarding interpretation criteria of CSF AD biomarkers is pivotal.Microcystinase (MlrA) was first described in 1996. Since then MlrA peptidase activity has proven to be both the most efficient enzymatic process and the most specific catalyst of all known microcystins detoxification pathways. Furthermore, MlrA and the MlrABC degradation pathway are presently the only enzymatic processes with clear genetic and biochemical descriptions available for microcystins degradation, greatly facilitating modern applied genetics for any relevant technological development. Recently, there has been increasing interest in the potential of sustainable, biologically inspired alternatives to current industrial practice, with note that biological microcystins degradation is the primary detoxification process found in nature. While previous reviews have broadly discussed microbial biodegradation processes, here we present a review focused specifically on MlrA. Following a general overview, we briefly highlight the initial discovery and present understanding of the MlrABC degradation pathway, before discussing the genetic and biochemical aspects of MlrA. We then review the potential biotechnology applications of MlrA in the context of available literature with emphasis on the optimization of MlrA for in situ applications including (i) direct modulation of Mlr activity within naturally existing populations, (ii) bioaugmentation of systems with introduced biodegradative capacity via whole cell biocatalysts, and (iii) bioremediation via direct MlrA application.Sludge is an inevitable by product of sewage treatment, and it includes pathogens, heavy metals, organic pollutants and other toxic substances. The components of sludge are complex and variable with extracellular polymeric substances (EPS) being one. EPS are highly hydrophilic and compressible, and make sludge dewatering difficult. Therefore, the development of efficient sludge-dewatering technology is an important means of mitigating rapid sludge growth. At present, the main methods used for sludge deep-dewatering technology are chemical preconditioning with high-pressure filtration and electrical mechanical dewatering. The selection of chemical preconditioning directly determines the final efficiency of the sludge-dewatering process. In this paper, we conduct a comprehensive review of the problems related to sludge dewatering and systematically summarise the impact of different chemical conditioning technologies on the efficiency of sludge dewatering.  https://www.selleckchem.com/products/cb-839.html  Furthermore, the characteristics of different enhanced dewatering technologies are evaluated and analysed for their adaptability and final disposal methods. We believe that this review can clarify the chemical conditioner mechanism to improve sludge dewatering, provide reference debugging information for the sludge-dewatering process and promote the development of efficient and environmentally friendly sludge-dewatering technology.Swampy/septic odor caused by various sulfides is one of the most frequently encountered odor problems in drinking water. However, even though it is much more offensive, few studies have specifically focused on swampy/septic odor compared to the extensively studied musty/earthy problems. In this work, four sulfide odorants, diamyl sulfide (DAS), dipropyl sulfide (DPS), dimethyl disulfide (DMDS) and diethyl disulfide (DEDS), were selected to evaluate the treatment performance of different oxidation processes in drinking water. The results demonstrated that DMDS, DEDS, DPS and DAS could be oxidized effectively by KMnO4, NaClO and ClO2. The oxidation processes could be well described by the second-order kinetic model, in which k values of selected sulfides followed the order DMDS≈DEDS ≪ DPS≈DAS. As for the three oxidants, the order of reactivity was KMnO4 ≪ ClO2 less then NaClO, which was also verified in raw water. The results of oxidation treatability, reaction kinetics and mechanisms confirmed that the characteristics of the central sulfur atom rather than the side chain is the decisive factor in controlling the oxidation rate and transformation pathway of sulfides. The transformation products and pathways were significantly different for the three oxidants. Sulfones (DPSO, DASO) were always formed by cycloaddition reactions during KMnO4 oxidation, yet recombination reactions proceeded during ClO2 oxidation and formed more products, such as MADS, DADS and EADS. Density functional theory (DFT) calculations confirmed that the differences in transformation pathways were caused by the variations in the activity of the oxidants and sulfides. Finally, NaClO was certified as the most effective oxidant for controlling sulfide odorants in drinking water treatment.The current Sphere guideline for water chlorination in humanitarian emergencies fails to reliably ensure household water safety in refugee camps. We investigated post-distribution chlorine decay and household water safety in refugee camps in South Sudan, Jordan, and Rwanda between 2013-2015 with the goal of demonstrating an approach for generating site-specific and evidence-based chlorination targets that better ensure household water safety than the status quo Sphere guideline. In each of four field studies we conducted, we observed how water quality changed between distribution and point of consumption. We implemented a nonlinear optimization approach for the novel technical challenge of modelling post-distribution chlorine decay in order to generate estimates on what free residual chlorine (FRC) levels must be at water distribution points, in order to provide adequate FRC protection up to the point of consumption in households many hours later at each site. The site-specific FRC targets developed through t health outcomes in refugee camp settings where the current Sphere chlorination guideline does not provide adequate residual protection. Water quality monitoring in refugee/IDP camps should shift focus from distribution points to household points of consumption in order to monitor if the intended public health goal of safe water at the point of consumption is being achieved.