Finally, we found that overexpressed LINC01939 inactivated Wnt/β-catenin and Notch through suppressing miR-154. Up-regulation of LINC01939 inhibited proliferation and migration of RCC cells by down-regulating miR-154.Objective We assessed (1) the factorial and convergent validity of the Portuguese version of the Stress Appraisal Measure (SAM); (2) maternal primary and secondary appraisals of the birth of a child; and (3) the influence of appraisals on stress.Background Parental stress is common during transition to parenthood and may be detrimental for parents and infants. Following Lazarus and Folkman's transactional model, the degree of stress is influenced by cognitive appraisals of an event, which are assessed by the SAM.Methods This was an observational, cross-sectional study. Two hundred and forty-five women with a child of up to 24 months of age filled out several self-report measures, in an online platform.Results The SAM factorial structure included three primary (Threat, Centrality, and Challenge) and two secondary (Controllable-by-Self and Controllable-by-Others) appraisals. All subscales had adequate internal consistency. The birth of a child was mainly perceived as a challenge, and seldom appraised as a threat. Threat and centrality appraisals positively predicted stress.Conclusion Assessing maternal appraisals of the birth of a child is relevant to foster adaptation to parenthood. The SAM is an adequate assessment tool. As cognitive appraisals are modifiable, women with higher threat appraisals should be targeted for psychological interventions.Background Obesity is strongly associated with exercise intolerance and development of heart failure. While myocardial energetics and diastolic function are impaired in obesity, systolic function is usually preserved. This suggests that the rate of ATP delivery is maintained, but this has never been explored in human obesity. We hypothesised that ATP transfer rate through creatine kinase (CK) (kf CKrest) would be increased, compensating for depleted energy stores (PCr/ATP), but potentially limiting greater ATP delivery during increased workload. We hypothesised these changes would normalise with weight loss. Methods We recruited 80 volunteers (35 controls (BMI 24±3kg/m2), 45 obese (BMI 35±5m/kg2)) without co-existing cardiovascular disease. Participants underwent body composition analysis, MRI of abdominal, liver and myocardial fat content, left ventricular function and 31P-magnetic resonance spectroscopy to assess PCr/ATP and CK kinetics, at rest and during dobutamine stress. Obese volunteers were assigned tery despite reduced PCr/ATP. During increased workload, whilst the non-obese heart increases ATP delivery through CK, the obese heart does not; this is associated with reduced systolic augmentation and exercise tolerance. Weight loss reverses these energetic changes. This highlights myocardial energy delivery via CK as a potential therapeutic target to improve symptoms in obesity-related heart disease, as well as a fascinating modifiable pathway involved in the progression to heart failure.Purpose Decompressive craniectomy (DC) is widely used to treat raised intracranial pressure (ICP) in cranial trauma and stroke. It is accompanied by numerous complications. The aim of our study is to assess the surgical treatment of infections related to the use of a dural substitute with concurrent CSF leakage performed at our institution.Material and methods A retrospective analysis of a series of 72 patients who underwent DC between 2011 and 2017 was performed. Seven cases (9%) showed infection related to the use of xenograft (bovine pericardium) and coexisting CSF leakage. Epidural/subdural empyemas were observed in seven cases; three in conjunction with an intracerebral abscess. For reconstruction, free anterolateral thigh fascia lata flaps were used, based on the size of the defect.Results After removal of the dural substitute and the implant of free fascia lata, infection and CSF leaks resolved in all. An anatomopathological examination of the implant at the later time of cranioplasty (CP) showed the tissue had become vascularized exhibiting integration with the native dura. No complications related to the harvesting of the fascia lata were observed.Conclusions Fascia lata is a validated source of autologous grafts; it is cost-free and would appear to be the biological material most similar to the dura mater. The implanted material appears to maintain a lasting vitality when covered over with a well-vascularized scalp, even after a period of months, achieving a successful suppression of infection. Subsequent skull reconstruction is performed safely and easily using artificial bone.To study the relationship between the expression of 10 selected genes in cumulus cells and the corresponding oocyte development competence, and the effect of patient age and body mass index on gene expression of cumulus cells, we collected 354 cumulus cell masses associated with individual oocyte from 48 women. The expression levels of the genes involved in glucose metabolism (PFKP, PKM2, LDHA and GFPT) and expansion (HAS2, VCAN, TNFAIP6, PTGS2, PTX3 and SDC4) in cumulus cells were detected by reverse transcription polymerase chain reaction. These were compared among oocyte maturity, fertilization, embryo morphology and implantation, and analyzed the effect of the subject's age and body mass index. Cumulus cell PFKP expression from mature oocytes was higher than those from immature oocytes (P = 0.014), and VCAN expression was higher from oocytes that developed into high-quality embryos (P = 0.024). TNFAIP6 expression in cumulus cells from fertilized oocytes was lower than that from unfertilized oocytes (P = 0on; GnRH gonadotropin-releasing hormone; hMG human menopausal gonadotropin; GV germinal vesicle; M I metaphase I; M II metaphase II; cDNA complementary DNA; SD standard deviation.Background and Objectives Having a stress-is-enhancing mindset - the extent to which one believes the effects of stress are enhancing rather than debilitating - is associated with greater health and well-being, and may mitigate negative outcomes associated with experiencing stress.  https://www.selleckchem.com/products/c646.html  The present study aimed to examine stress mindset and whether it moderates the association between perceived stress and mental health outcomes.Design and Methods Participants (N = 293) completed questionnaires assessing their stress mindset, perceived stress level, and current depressive and anxiety symptoms. Perceived stress and mental health were assessed again at a follow-up session one month later.Results Across moderated linear regression analyses, there was a consistent pattern in which higher perceived stress was associated with higher mental health concerns, but the risk was greater for those with a stress-is-debilitating mindset. Stress mindset moderated the perceived stress-depression association at baseline (p = .026), at follow-up (p = .