Enzymatic biofuel cell (EBFC)-based self-powered biochemical sensors obviate the need for external power sources thus enabling device miniaturization. While recent efforts driven by experimentalists illustrate the potential of EBFC-based sensors for real-time monitoring of physiologically relevant biochemicals, a robust mathematical model that quantifies the contributions of sensor components and empowers experimentalists to predict sensor performance is missing. In this paper, we provide an elegant yet simple equivalent circuit model that captures the complex, three-dimensional interplay among coupled catalytic redox reactions occurring in an EBFC-based sensor and predicts its output signal with high correlations to experimental observations. The model explains the trade-off among chemical design parameters such as the surface density of enzymes, various reaction constants as well as electrical parameters in the Butler-Volmer relationship. The model shows that the linear dynamic range and sensitivity of the EBFC-based sensor can be independently fine-tuned by changing the surface density of enzymes and electron mediators at the anode and by enhancing reductant concentrations at the cathode. The mathematical model derived in this work can be easily adapted to understand a wide range of two-electrode systems, including sensors, fuel cells, and energy storage devices.Fish freshness plays a vital role in the fish industry and also affects human health from a nutrition point of view. Here, an Au metal patch electrode capacitive sensor is introduced for rapid and accurate detection of volatile gases generated from raw fish to determine its freshness status. The MIS structured sensor was fabricated on a silicon substrate using Ag-SnO2 as sensing material over SiO2 layer and Au as a metal electrode. The calibration of the sensor was carried out with known composition of volatile gases (NH3, TMA, DMA and H2S) in the ppb-ppm regime. Our sensor using the proposed technique delivers sensor response to raw fish within 4 min, reflecting its freshness status in comparison to the TVB-N and TVC method which takes many hours to complete involving many sophisticated steps. The sensor response to volatile gases from Rohu (Labeo Rohita), Tilapia (Oreochromis Niloticus) and Illish (Tenualosa Ilisha) obtained by using our developed sensor system at 20 °C, 25 °C, and 30 °C, shows high correlation with TVB-N and TVC results. The acceptance limit for safe consumption of Tilapia, Rohu, and Illish at 30 °C was found to be 11 h, 12.5 h, and 10 h of storage time respectively. The result in this work assures a portable low-cost sensor for onsite monitoring of fish freshness at room temperature.Low grade appendiceal mucinous neoplasm (LAMN) is the primary source of pseudomyxoma peritonei (PMP). PMP may develop after seemingly complete resection of primary tumor by appendectomy, which is unpredictable due to lack of reliable prognostic indicators. We retrospectively reviewed 154 surgically resected LAMNs to explore if any of the macroscopic and microscopic characteristics may be associated with increasing risk of PMP development.  https://www.selleckchem.com/products/Temsirolimus.html  Our major findings include (1) As compared to those without PMP, the cases that developed PMP were more frequent to have (a) smaller luminal diameter ( less then 1 cm) and thicker wall, separate mucin aggregations, and microscopic perforation/rupture, all suggestive of luminal mucin leakage; (b) microscopic acellular mucin presenting on serosal surface and not being confined to mucosa; and (c) neoplastic epithelium dissecting outward beyond mucosa, however, with similar frequency of neoplastic cells being present in muscularis propria. (2) Involvement of neoplastic cells or/and acellular mucin at surgical margin did not necessarily lead to tumor recurrence or subsequent PMP, and clear margin did not absolutely prevent PMP development. (3) Coexisting diverticulum, resulted from neoplastic or non-neoplastic mucosa being herniated through muscle-lacking vascular hiatus of appendiceal wall, was seen in a quarter of LAMN cases, regardless of PMP. The diverticular portion of tumor involvement was often the weakest point where rupture occurred. In conclusion, proper evaluation of surgical specimens with search for mucin and neoplastic cells on serosa and for microscopic perforation, which are of prognostic significance, should be emphasized.High-grade diffuse large B-cell lymphoma (HG-DLBCL) refers to DLBCL with MYC and BCL2 and/or BCL6 rearrangements (double-hit or triple-hit DLBCL) that exhibits poor prognosis. Double-expressor DLBCL (c-myc+/bcl-2+) has intermediate prognosis when compared to HG-DLBCL. Primary central nervous system lymphoma (PCNSL) has distinct pathophysiology (frequent non-germinal center-like subtype and double-expressor) and has worse prognosis than systemic DLBCL. By fluorescence in situ hybridization (FISH), 25-30% of PCNSLs harbor BCL6 abnormalities with rare alterations in MYC, BCL2, double-hit or triple-hit events. We describe the clinicopathologic features and status of MYC, BCL2 and BCL6 in 12 PCNSLs (7 women, 5 men; median age 63 years; range 28-79). Six cases showed focal starry-sky pattern. Immunohistochemically, all (100%) were of non-germinal center-like subtype, and 8/10 (80%) cases were double-expressors. Ki-67 ranged from 70 to 100%. FISH was positive in 9/12 (75%) cases 4 (33%) harbored a BCL6 rearrangement, 3 (25%) had a gain of BCL2, 2 (17%) cases each had a gain of BCL6 and gain of IGH, and gain of MYC and deletion of MYC were observed in 1 case each (8%). Two (16%) cases were MYC/BCL6 double-hit PCNSLs. No MYC/BCL2 or triple-hit cases were identified. Eleven (92%) patients received chemotherapy and one also received whole brain radiation. The median time of follow-up was 4.4 months (range, 0.3-40.3). Seven (58%) patients are alive, 4 (33%) have died, and 1 (8%) had no follow-up. Five alive patients are in remission, including one MYC/BCL6 double-hit PCNSL. Our results add two new cases of rare double-hit PCNSL to the literature.Socioeconomic status (SES) as well as adolescents' perceptions of the neighborhood's built and social environments have been shown to influence adolescents' physical activity. Twenty-four adolescents from two low SES neighborhoods in Sweden participated as citizen scientists, using the Stanford Discovery Tool app on their phones to take photographs and record audio narratives of aspects of their neighborhood that they perceived facilitate or hinder their physical activity. The most frequently reported facilitators were 'parks, playgrounds and outdoor gym' as well as 'amenities' and 'sport facilities', whereas lack of or shortcomings regarding 'bikeability and walkability', 'personal safety' and 'lighting' were the most frequently reported barriers. The results will be used to inform local politicians and policy makers about new ways to improve physical activity among residents in Sweden's low SES neighborhoods. Also, this study shows that a Swedish version of the Discovery Tool app is acceptable and can generate useful information in the context of adolescents from disadvantaged neighborhoods.