024) when compared to two doses of NaFl. There was no association with age, or dry eye signs or symptoms on the variance observed in any of the indices between baseline, intervention I, and intervention II (P > 0.05). Agreement between corneal surface indices reduced following the addition of NaFl. CONCLUSION In comparison to measurements taken without an ocular dye, one dose of NaFl resulted in increased reliability and consistency in corneal topography measurements using the E300 topographer, but 2 doses decreased reliability and consistency. Practitioners ought to be aware that tear film surface regularity and inferior-superior corneal power changed significantly following the addition of NaFl in those with healthy corneas. Its effect in diseased corneas is unknown.  https://www.selleckchem.com/products/Nolvadex.html  PURPOSE To demonstrate the necessity of aligning a wavefront-guided scleral lens (WGSL) optical correction to the eye's effective pupil, with misalignments leading to reduced performance. CASE REPORT A 34 year old subject with a history of failed LASIK in the left eye, leading to penetrating keratoplasty, extracapsular extraction of the crystalline lens and neodymiumyttrium-aluminum-garnet (NdYAG) laser posterior capsulotomy, enrolled in a study examining WGSL performance. Habitual logMAR acuity OS (aided with a scleral lens) was +0.04. Residual higher order root mean square (HORMS) wavefront error (WFE) was 0.28 μm (Φ =4.75 mm, mean age-matched norm =0.17 μm), and objective over-refraction was -0.30 -0.54 × 008. When a WGSL (targeting aberrations up to the 5 th radial order) was manufactured with the wavefront-guided optics aligned to the center of the dilated pupil, logMAR acuity worsened to +0.15, residual HORMS WFE worsened to 0.44 μm (Φ =4.75 mm), and objective over-refraction increased to +1.19 -0.30 × 122. Slit lamp imagery revealed that the effective pupil was no longer defined by the iris of the eye, but rather the capsular opening created by the capsulotomy. When the WGSL was redesigned to align the wavefront-guided optics to the center of the capsular opening, logMAR acuity improved to -0.14, residual HORMS WFE reduced to 0.17 μm (Φ =4.75 mm) and objective over-refraction reduced to +0.20 -0.15 × 111. CONCLUSION WGSLs are an emerging option for patients with highly aberrated, ectatic and post-surgical corneas whose visual symptoms cannot be alleviated with conventional corrections. However, alignment of the optics of the WGSL to the underlying optics of the eye over the effective pupil is critical in achieving good optical and visual performance. The nasal columella is often described as being one of the most difficult nasal subunits to reconstruct. There are a wide range of indications for columella reconstruction, with defects resulting from ischaemic injuries, trauma, tumour resection, vascular malformations and congenital agenesis/dysgenesis of nasal anatomy. There is a variety of columella reconstruction techniques reported in the literature, giving reconstructive surgeons options when approaching different columella defects. Each technique has surgical pearls and pitfalls as well as advantages and disadvantages. This review aims to give reconstructive surgeons a comprehensive review of currently used columella reconstruction techniques. Crown All rights reserved.INTRODUCTION The flap necrosis rate remains high despite the advancement of technology in daily practices. Several randomized trials of topical Nitroglycerin (NTG) have shown promise in reducing flap necrosis. We aim to evaluate the efficacy and safety of topical NTG in preventing flap failure based on existing databases. METHOD We searched through PubMed, EuropePMC, EBSCOhost, Cochrane CENTRAL database, Clinicaltrials.gov, and hand sampling for "flap survival", "topical nitroglycerin", and "flap perfusion". RESULTS With a total of 6947 patients from 3 RCT and 2 retrospective cohorts, NTG was shown to prevent flap failure in mastectomy flaps by NTG with an OR 0.23 [0.10, 0.53]; p less then 0.001), I2 73%. Upon sensitivity analysis to reduce heterogeneity, the OR was 0.17 [0.07, 0.40]; p less then 0.001, I2 52%. Upon subgroup analysis of RCT, the OR was 0.17 [0.10, 0.30]; p less then 0.001, I2 50%. Newer studies subgroup had OR 0.48 [0.33, 0.70]; p less then 0.001; I2 46. Upon subgroup analysis of single application only, the OR for flap necrosis was 0.36 [0.18, 0.73]; p = 0.005, I2 67% and subgroup analysis repeated application had an OR of 0.05 [0.01, 0.21]; p less then 0.001, I2 14%. CONCLUSION Nitroglycerin seemed to be an ideal agent to increase the chance of flap survival in mastectomy flaps. It has an excellent safety profile, hence, is suitable for empiric use. More randomized controlled trials comparing different regiments and other preparations are needed to conclude whether repeated application at a low dose is most effective, and whether the success is reproducible on other types of flaps. The surgical treatment of Charcot foot is a widely debated topic, with issues ranging from when to operate to how to properly correct a deformity. Historically, correction of a severe deformity was attempted in 1 acute surgical procedure that frequently required open reduction and internal fixation through large incisions. This 1-time procedure would often result in complications including under- or overcorrection of the deformity, neurovascular injury, or incision dehiscence leading to possible soft-tissue infection or osteomyelitis. This retrospective case series aims to evaluate stage 1 of a planned 2-stage approach to Charcot deformity correction, consisting of gradual modification with the use of computer-assisted external fixation. The purpose of using gradual correction was to safely and accurately correct the Meary and calcaneal inclination angles, which were measured using preoperative and postoperative digital radiographs. The procedure was performed on 18 Charcot foot deformities in 18 patients. Each of the feet had a notably significant rocker bottom deformity and most contained an ulceration. Complete ulcer healing was noted in 100% (13/13) of feet with an ulcer, and a statistically significant corrected Meary's (p less then .05) and calcaneal inclination angle (p less then .05) to within a normal range was achieved in all deformity corrections with few postoperative problems and complications noted. Average patient follow-up was 39.6 months with a minimum of at least 12 months necessary for inclusion in the study. Therefore, gradual Charcot deformity correction through the use of computer-assisted hexapod external fixation, demonstrates safe, accurate, and reproducible characteristics that adequately prepares the lower extremity for stage 2, the implantation of rigid internal fixation.