It was evident that the reverse micelles diameter was linearly proportionated to the amount of laccase enzyme incorporated. BPA bioremediation mediated by laccase in non-aqueous environment was found to be 84% in 8 h of treatment. Biotransformation of BPA was monitored using GC-MS. BPA degraded products, such as BPA-O-catechol and 4,4 (Ethane 2-oxy 2-ol) diphenol were identified indicating transformation by oxidation.
  The online version contains supplementary material available at 10.1007/s13205-021-02842-4.
 The online version contains supplementary material available at 10.1007/s13205-021-02842-4.Algae have gained substantial importance as the most promising potential green fuel source across the globe and is on growing demand due to their antioxidant, anticancer, antiviral, antihypertensive, cholesterol reducing and thickening properties.  https://www.selleckchem.com/products/gsk046.html  Therefore, it has vast range of application in medicines, pharmaceutical, cosmetics, paper and nutraceutical industries. In this work, the remarkable ability of algae to convert CO2 and other toxic compounds in atmosphere to potential biofuels, foods, feeds and high-value bioactive compounds is reviewed. Algae produce approximately 50% of the earth's oxygen using its photosynthetic activity, thus acting as a potent tool to mitigate the effects of air pollution. Further, the applicability of algae as a desirable energy source has also been discussed, as they have the potential to serve as an effective alternative to intermittent renewable energy; and also, to combustion-based fossil fuel energy, making them effective for advanced biofuel conversions. This work also evaluates the current applications of algae and the implications of it as a potential substrate for bioplastic, natural alternative to inks and for making paper besides high-value products. In addition, the scope for integrated biorefinery approach is also briefly explored in terms of economic aspects at the industrial scale, as such energy conversion mechanisms are directly linked with sustainability, thus providing a positive overall energy outlook.D-pantothenic acid (D-PA), as a crucial vitamin, is widely used in food, animal feed, cosmetics, and pharmaceutical industries. In our previous work, recombinant Escherichia coli W3110 for production of D-PA was constructed through metabolic pathway modification. In this study, to enhance D-PA production, statistical optimization techniques including Plackett-Burman (PB) design and Box-Behnken design (BBD) first were adopted to optimize the culture condition. The results showed that the glucose, β-alanine and (NH4)2SO4 have the most significant effects on D-PA biosynthesis. The response surface model based on BBD predicted that the optimal concentration is glucose 56.0 g/L, β-alanine 2.25 g/L and (NH4)2SO4 11.8 g/L, the D-PA titer increases from 3.2 g/L to 6.73 g/L shake flask fermentation. For the fed-batch fermentation in 5 L fermenter, the isoleucine feeding strategy greatly increased the titer and productivity of D-PA. As a result, titer (31.6 g/L) and productivity (13.2 g/L·d) of D-PA were achieved, they increased by 4.66 times and 2.65 times, respectively, compared with batch culture. At the same time, the accumulation of acetate reduced from 29.79 g/L to 8.55 g/L in the fed-batch fermentation. These results demonstrated that the optimization of medium composition and the cell growth rate are important to increase the concentration of D-PA for microbial fermentation. This work laid the foundation for further research on the application of D-PA microbial synthesis.
  The online version contains supplementary material available at 10.1007/s13205-021-02773-0.
 The online version contains supplementary material available at 10.1007/s13205-021-02773-0.Cyst nematodes of the species Globodera rostochiensis and G. pallida are devastating parasites of the potato crop. Early detection of cyst nematodes in the field is critical for adopting an appropriate management strategy. A specific and sensitive loop-mediated isothermal amplification (LAMP) assay using four oligonucleotide primers has been developed to amplify the internal transcribed spacer region (ITS) of ribosomal DNA of potato cyst nematode G. rostochiensis. The PCN-LAMP reaction could be completed within 75 min at 68 °C followed by termination at 85 °C for 7 min. The primers exhibited specificity for G. rostochiensis and did not detect any other tested genera of plant parasitic or entomopathogenic nematodes. LAMP reaction was highly sensitive, suitable for crude genomic DNA and could successfully detect G. rostochiensis DNA up to femtogram quantity. This assay is rapid, cost effective and requires minimal instrumentation. It will facilitate the detection of G. rostochiensis at field and point-of-care labs and help in the interception of infested plant material/soil samples at quarantine stations independent of a professional nematologist.
  The online version contains supplementary material available at 10.1007/s13205-021-02830-8.
 The online version contains supplementary material available at 10.1007/s13205-021-02830-8.Conventional agricultural practices based on the application of synthetic fertilizers are increasingly considered as unsustainable. Under a forecasted scenario of drought for the next decades, there is a global demand for innovative and sustainable approaches to ameliorate plant performance. Here, encapsulating beneficial microbes (BMs) to promote plant growth is gaining attention. This study evaluates bacterial encapsulation using polymeric beads of alginate, testing the survival of Pseudomonas libanensis TR1 stored up to 90 days. Produced beads were subjected to different treatments (fresh, air-dried and pulverized), which resulted in a variable size range (1200-860 µm). After storage, bacterial viability was maintained, and air-dried beads displayed a higher number of colony-forming units (2 × 107). Then, a glasshouse experiment investigated the drought resistance (plant growth, biomass, and photosynthetic responses) of Vigna unguiculata plants inoculated with these alginate beads. After 10 days of complete water restriction, turgidity and relative water content of V.