https://bay2666605inhibitor.com Higher plant photosynthesis relies on crucial photosynthetic pigments, including chlorophyll (Chl) and carotenoids, which also play a protective role against light damage. In chlorophyll-deficient tobacco seedlings, photosynthesis tends to be less efficient, and the concentration of nitrate-nitrogen (NO3-) is usually higher. Tobacco yield and quality are contingent upon the N) content. In this research, the common flue-cured tobacco cultivar 'Zhongyan 100' (ZY100) provided a stable albino leaf mutant (Al) and a slight-green leaf mutant (SG), obtained through mutagenesis using ethyl methanesulfonate (EMS). An analysis of biomass, photosynthetic fluorescence parameters, and carbon/nitrogen-related physiological aspects was undertaken to discern the distinctions between the Chl-deficient mutants and the wild-type (WT). Applying RNA sequencing (RNA-seq) and weighted gene co-expression network analysis (WGCNA), the research investigated the key pathways and candidate genes controlling the differential content of chlorophyll and nitrate. The study's findings demonstrated that mutant plants had considerably lower chlorophyll content and biomass when evaluated against wild-type (WT) controls, while also showing a decrease in NO levels. N content levels were substantially heightened. Mutant leaf analyses revealed decreased photosynthetic rates, fluorescence parameters, carbohydrate amounts, soluble protein levels, and activities of carbon and nitrogen-related enzymes; chloroplast development was also abnormal. Mutants displayed augmented growth and development with the administration of extra nitrogen, in contrast to the presence of NO's influence. The concentration of N content demonstrably elevated when contrasted with the WT. Analysis of transcriptomes from mutant plants indicated that genes associated with plant hormone signaling cascades and nitrogen utilization were disproportionately downregulated, impacting pigment synthesis and carbon