https://www.selleckchem.com/products/cpi-203.html 20-fold and ~2.13-fold higher than the G/CNF film, respectively). Also, G/CNF with 3% w/w ZnO NPs (G/CNF/ZnO2) film had the lowest water vapor permeability and water solubility among all films. Results of the disc diffusion assay showed a stronger antibacterial effect of ZnO NPs compared with Se NPs. The bacterial susceptibility to the antibacterial films was as follows Listeria monocytogenes > Escherichia coli > Staphylococcus aureus > Pseudomonas fluorescens. The G/CNF films incorporated with Se nanoparticles possessed the higher property of scavenging free radicals in comparison films containing ZnO nanoparticles. Also, the combination of Se NPs and ZnO NPs enhanced the antioxidant effect of the films. In conclusion, gelatin-based edible films containing CNFs, ZnO NPs, and Se NPs can be used in the development of active food packaging products.Compression-decompression surface pressure (π)-specific molecular area (A) isotherm cycle of human serum albumin (HSA) monolayer is performed on water surface at four different subphase pH conditions, i.e., below and above the isoelectric point (pI ≈ 4.7) of HSA molecule. For all pH conditions, the decompression curve nearly follows the compression curve, however, at pH ≈ 5.0, hysteresis is observed at higher surface pressure. Out-of-plane structures and in-plane morphologies obtained from the X-ray reflectivity and AFM studies show that only the film thickness variation takes place with the change in surface pressure, which is also evidenced from the BAM images. With increase in surface pressure, the oblate-shaped HSA molecules start tilting making an angle with the water surface and as the monolayer is decompressed the molecules regain their initial untilted monomolecular configuration. Depending upon the subphase pH and local surface charge of the specific protein molecule, electrostatic repulsive interaction dominates over the van der Waals attraction and as a result dec