https://www.selleckchem.com/products/ccs-1477-cbp-in-1-.html platensis biomass. This study aimed to evaluate the potential of live and lyophilized fungi-algae pellets as biosorbents for gold recovery and their regeneration ability. The optimum conditions determined by Taguchi method were 1 g/L co-pellets, 9-10 mm size at 250 rpm of agitation speed and pH 3.5 and 2.0 for live and lyophilized co-pellets, respectively. The porous characteristics of fungi-algae pellets played an important role on gold adsorption. Lyophilized co-pellets achieved adsorption capacity of 112.36 mg/g which were comparable with some synthesized granular adsorbents and performed better than the live co-pellets due to more cell-wall polysaccharides involved in gold interaction. 97.77% of gold was selectively absorbed by the lyophilized co-pellets from multi-metal wastewater in column reactor. This study may provide new insights into the application of fungi-algae pelletized reactor in bioremediation of contaminated wastewater by precious metals and their recovery and the in-situ regeneration of biosorbents. Two full-scale high-rate bioreactors, i.e. external circulation sludge bed (ECSB) and expanded granular sludge bed (EGSB), were monitored for three years. Their performances for treating wastewater in a whiskey distillery were compared in terms of COD, pH, alkalinity and VFA. Even though feed flowrate highly fluctuated, COD removals of ECSB and EGSB were both excellent (95.7 ± 1.3% and 94.8 ± 3.0%, respectively). The influent and effluent characteristics of ECSB reactor were profiled and urea and urethane were also detected. High-strength properties of raw spent wash were exhibited in TOC, soluble COD and BOD5,20°C of 13500, 37750, and 1950 mg·L-1, respectively and characterized by GC-MS. Anaerobic granular sludge sampled from different heights of ECSB reactor were fractionated for demonstrating vertical size distributions. Moreover, major species found by next-generation sequencing techniqu