The results of He atom scattering experiments on KTa0.48Nb0.52O3(001) surfaces are presented and compared with similar experiments on mixed potassium tantalate/niobate perovskites with lower concentrations of Nb. The results are puzzling, unique and intriguing. Angular distributions of the He scattering intensities (the He surface diffraction pattern) are found to be very similar to those obtained from targets with lower Nb fractions. However, drift spectra (the intensity of the He specular reflection as a function of the He wave vector) are not. Whereas the drift spectra in the 〈10〉 azimuth do resemble those of the 0, 6 and 10% Nb fraction targets, in the 〈11〉 azimuth they are more similar to the strange drift spectra found from the 30% Nb-doped targets. Most intriguing are the surface temperature scans (the He specular intensity as a function of the target surface temperature); for they are quite distinct from those with lower Nb fractions. Finally, the inelastic He scattering experiments provide phonon dispersion results that are similar in most respects to those found for the lower Nb level samples. In particular, the most prominent feature is an Einstein-like mode at about 13.5 meV which spans the entire surface Brillouin zone in both 〈10〉 and 〈11〉 azimuths.Functional molecular groups mounted on specific foot structures are ideal model systems to study intermolecular interactions, due to the possibility to separate the functionality and the adsorption mechanism. Here, we report on the rotational switching of a thioacetate group mounted on a tripodal tetraphenylmethane (TPM) derivative adsorbed in ordered islands on a Au(111) surface. Using low temperature scanning tunnelling microscopy, individual freestanding molecular groups of the lattice can be switched between two bistable orientations. The functional dependence of this rotational switching on the sample bias and tip-sample distance allows us to model the energy landscape of this molecular group as an electric dipole in the electric field of the tunnelling junction. As expected for the interaction of two dipoles, we found states of neighbouring molecules to be correlated.The Golden Gate cloning method enables the rapid assembly of multiple genes in any user-defined arrangement. It utilizes type IIS restriction enzymes that cut outside of their recognition sites and create a short overhang. This modular cloning (MoClo) system uses a hierarchical workflow in which different DNA parts, such as promoters, coding sequences (CDS), and terminators, are first cloned into an entry vector. Multiple entry vectors then assemble into transcription units. Several transcription units then connect into a multi-gene plasmid. The Golden Gate cloning strategy is of tremendous advantage because it allows scar-less, directional, and modular assembly in a one-pot reaction. The hierarchical workflow typically enables the facile cloning of a large variety of multi-gene constructs with no need for sequencing beyond entry vectors. The use of fluorescent protein dropouts enables easy visual screening. This work provides a detailed, step-by-step protocol for assembling multi-gene plasmids using the yeast modular cloning (MoClo) kit. We show optimal and suboptimal results of multi-gene plasmid assembly and provide a guide for screening for colonies. This cloning strategy is highly applicable for yeast metabolic engineering and other situations in which multi-gene plasmid cloning is required.Network Function Virtualization (NFV) has been regarded as one of the key enablers for the 5th Generation of mobile networks, or 5G. This paradigm allows to reduce the dependence on specialized hardware to deploy telecommunications and vertical services. To this purpose, it relies on virtualization techniques to softwarize network functions, simplifying their development and reducing deployment time and costs. In this context, Universidad Carlos III de Madrid, Telefónica, and IMDEA Networks Institute have developed an NFV ecosystem inside 5TONIC, an open network innovation center focused on 5G technologies, enabling the creation of complex, close to reality experimentation scenarios across a distributed set of NFV infrastructures, which can be made available by stakeholders at different geographic locations. This article presents the protocol that has been defined to incorporate new remote NFV sites into the multi-site NFV ecosystem based on 5TONIC, describing the requirements for both the existing and the newly incorporated infrastructures, their connectivity through an overlay network architecture, and the steps necessary for the inclusion of new sites. The protocol is exemplified through the incorporation of an external site to the 5TONIC NFV ecosystem. Afterwards, the protocol details the verification steps required to validate a successful site integration. These include the deployment of a multi-site vertical service using a remote NFV infrastructure with Small Unmanned Aerial Vehicles (SUAVs). This serves to showcase the potential of the protocol to enable distributed experimentation scenarios.Acute kidney injury (AKI) is associated with higher risk for morbidity and mortality post-operatively. Ischemia-reperfusion injury (IRI) is the most common cause of AKI. To mimic this clinical scenario, this study presents a highly reproducible large animal model of renal IRI in swine using temporary percutaneous bilateral balloon-catheter occlusion of the renal arteries. The renal arteries are occluded for 60 min by introducing the balloon-catheters through the femoral and carotid artery and advancing them into the proximal portion of the arteries.  https://www.selleckchem.com/products/sndx-5613.html  Iodinated contrast is injected in the aorta to assess any opacification of the kidney vessels and confirm the success of the artery occlusion. This is furtherly confirmed by the flattening of the pulse waveform at the tip of the balloon catheters. The balloons are deflated and removed after 60 min of bilateral renal artery occlusion, and the animals are allowed to recover for 24 h. At the end of the study, plasma creatinine and blood urea nitrogen significantly increase, while eGFR and urine output significantly decrease.