Soil surface microtopography plays a significant role in rill erosion. In addition, upslope inflow has a large effect on downslope soil erosion processes. Experiments including four different upslope filling areas (0 m2, 0.15 m2, 0.30 m2, and 0.45 m2) with a upslope inflow rate (6 L min-1) were conducted in two 1 m × 2 m boxes on a 15° slope to examine the effects of microtopography and sediment concentration on rill erosion processes. The upslope filling areas were used to simulate different areas of earthen dike terraces. The results showed that the minimum values of soil surface elevation increased from -120 mm to -110 mm as the upslope filling area increased. The values of the simple fractal dimension (f(α)max), the singular index span (Δα) and the difference of multifractal spectrum (Δf(α)) reached minimum values in the 0.45 m2 upslope filling area. With the development of rill erosion, the soil surface microtopography tended to sharpen, and the relative elevation changed greatly. The runoff and soil loss associated with rill erosion gradually decreased as the upslope filling area increased. We identified the temporal evolution of rill erosion using Morlet wavelet analysis.  https://www.selleckchem.com/products/propionyl-l-carnitine-hydrochloride.html  The main period of temporal fluctuation of rill erosion was 28 min under different upslope filling areas. Multi-scale periods of temporal fluctuation of rill erosion emerged with the increase in upslope filling area. The Δα significantly affected the runoff and soil loss. The proportional contributions of Δα to the runoff and sediment yield were 80.95% and 77.34%, respectively. While the contributions of sediment concentration to runoff and sediment yield were 17.05% and 20.66%, respectively. The findings are important significance for better understanding rill erosion mechanisms of purple soil.Antibiotic residues in mariculture environments have been detected globally, while little information is available about their dynamic levels, source, behavior, and fate during the whole culture process. In this study, the dynamic occurrence, bioaccumulation, source, fate, and human dietary risk of 19 antibiotics were investigated in different breeding stages of mariculture ponds near the Maowei Sea, South China. Fourteen antibiotics, including three sulfonamides (SAs), five fluoroquinolones (FQs), three macrolides (MLs), and two chloramphenicols (CAPs), were detected in the mariculture ponds, with FQs being the most abundant antibiotics. Significant variations of antibiotic concentration occurred during the whole culture process. Severe weather, especially typhoons and rainstorms, resulted in the average highest levels of ∑19antibiotics (mean 567 ng L-1) in mariculture ponds. The source apportionment estimated for the mariculture ponds showed that direct application was the primary source of antibiotics (91.2%). The antibiotics in mariculture ponds were mainly discharged through aquaculture wastewater (65.8%) and settling particles (33.8%). The estimated annual input of antibiotics into the Maowei Sea was 2.24 times higher through the two main rivers (48.0 kg a-1) than through the mariculture wastewater (24.1 kg a-1). The apparent bioaccumulation factors (ABAFs) confirmed that young and adult tilapia accumulated more sulfamethoxazole (SMX) and norfloxacin (NOX), respectively. The result from the estimated daily intakes suggested that the antibiotics in the seafood could not pose a risk to human health by dietary exposure assessment. CAPSULE Big variation of antibiotic concentration occurred during the whole culture process in the mariculture farms, and the storm increased antibiotic application.Recent studies have shown that free nitrous acid (FNA, i.e., HNO2) is biocidal to many microorganisms, promoting the development of FNA-based technology in biological wastewater treatment. Suppression of nitrite-oxidizing bacteria (NOB) is a critical step for autotrophic nitrogen removal via anammox. In this study, the biocidal effect of FNA on NOB was determined by developing a model methodology combined with NOB incubation. Sixteen groups of FNA exposure tests were conducted at five different FNA concentrations from 0 to 4 mg HNO2-N/L, obtained from three pH values (5.0, 5.5 and 6.0) with nitrite ranged from 21 to 1680 mg NO2--N/L, with one as a control. Nitrate production curves were tracked during incubations of the FNA-exposed sludge, and then used to estimate active NOB concentrations by the kinetic model-based fitting. The results showed that with 24-hour exposure to FNA at a level of over 1 mg HNO2-N/L, the active NOB decreased around two orders of magnitude compared with that in the primordial sludge. The Weibull model can well describe the biocidal effect, which would be useful for the optimization of FNA conditions. The maximum NOB growth rate was increased after FNA exposure. This result suggests that long-term implementation of FNA-based technology can select fast-growing NOB in activated sludge, causing a 'NOB adaptation' issue.Soil fauna takes an active part in accelerating turnover of nutrients in terrestrial ecosystems. Anaerobic ammonium oxidation (anammox) has been widely characterized, however, whether anammox is active in earthworm gut and the effect of earthworm on anammox in soil remain unknown. In this study, the activity, abundance and community of anammox bacteria in earthworm guts and soils from microcosms were determined using a 15N-tracing technique, quantitative PCR, and anammox bacterial 16S rRNA gene amplicon sequencing. Results showed that anammox rates in guts ranged between 5.81 and 14.19 nmol N g-1 dw gut content h-1, which were significantly (P less then 0.01) higher than that in their surrounding soils during 30 day incubation. On the contrary, abundances of hzsB genes encoding subunit B hydrazine synthase in guts were significantly (P less then 0.05) lower than those in their surrounding soils. Anammox rates, denitrification N2 production rates and hzsB genes in soils with earthworms were significantly (P less then 0.05) lower than those in control soils. Anammox bacterial compositions differed significantly (P less then 0.05) between gut and soil, and earthworm altered anammox bacterial communities in soils. Brocadia, Kuenenia and abundant unclassified anammox bacteria were detected in collected soils and gut contents, in which Brocadia was only detected in guts. These results suggested that microbes in earthworm gut increase, but present of earthworm reduces anammox and denitrification associated N loss by altering the anammox bacterial community compositions in soils.