During torsion stress simulation a decrease of the value of the maximal stress in the lateral hinge was also observed with the addition of a hinge-securing screw (37 MPa without screw, 27Mpa with a 2 mm screw and 25 Mpa with a 4 mm screw). INTERPRETATION Positioning a screw intersecting the cutting plane at the theoretical lateral hinge location associated with a locking plate reduces lateral hinge stress in both compression and torsion. Those findings need to be confirmed by further specimens' mechanical testing. Paeonia ostii is an emerging woody oil crop and cultivated on a large scale in China. This study attempted to characterize the characteristics of P. ostii seed oil body (OB) and found the key genes related to its morphology. The results showed that P. ostii seed oil contained five dominant fatty acid compositions that were stored in OBs. During the development of seeds, oil yield and fatty acid content were in line with OB accumulation. Moreover, NaCl concentration and pH value could influence P. ostii OB stability. Additionally, oleosins (OLEs) were identified as the most abundant proteins in OBs. Based on the expression levels of OLEs, PoOLE17.5 was isolated, which might be localized in OBs. And overexpression of PoOLE17.5 in tobacco obviously increased seed size and hundred-seed weight, altered OB morphology and increased fatty acid content. These results could provide a theoretical basis for improving oil yield of P. ostii seeds.  https://www.selleckchem.com/PI3K.html  Colorimetric biosensors have been widely applied to mycotoxins testing. However, the colorimetric assay previously reported used a single color to detect one mycotoxin, and there were few reports on the simultaneous detection of multiple mycotoxins. In this work, a colorimetric biosensor for dual mycotoxins detection was developed. A Fe3O4/GO based platform for aflatoxins B1 (AFB1) detection and a Fe3O4@Au based platform for ochratoxin A (OTA) detection were fabricated. The quantification of OTA and AFB1 was respectively achieved by the release of thymolphthalein under alkaline conditions and 3,3',5,5'-tetramethylbenzidine was catalyzed by Au NPs under acidic conditions. Because of different conditions, two sensing methods didn't interfere with each other but could provide a higher detection efficiency. The detection range of AFB1 is 5-250 ng·ml-1 and that of OTA is 0.5-80 ng·ml-1. This biosensor has been successfully applied in real sample detection, which has a broad application prospect in fields of food safety. Simple, fast and efficient method for on-site detection of meat adulteration is of increasing demand in low-resource regions. Here, a direct and visual denaturation bubble-mediated strand exchange amplification method (SEA) was developed to detect meat adulteration by targeting the mitochondrial sequence from duck, which required only a heating block. The method allowed detecting as low as 10 pg/μL duck DNA and 0.1% duck meat in binary mixtures, sufficiently meeting the demand of meat adulteration detection. Notably, the method realized instrument-free readout by significant color discrimination of positive and negative results. By coupling with fast DNA release, a direct SEA method was developed and reduced the whole detection time to 1 h, eliminating complex DNA extraction process. The developed method provided a promising strategy with integration of fast sample lysis, miniaturized reaction platform and visual readout for meat adulteration, especially suitable for low resource point-of-care settings. SeMet-Pro-Ser (Se-MPS) is an antioxidant selenopeptide derived from selenized brown rice protein hydrolysates. In this study, the stability of glutathione (GSH), Met-Pro-Ser (MPS), and Se-MPS under different processing conditions, namely, temperature, pH, NaCl, citric acid, light, and gastrointestinal proteases, were evaluated by measuring ABTS+ scavenging and Cr(VI) reduction capacities. The ABTS+ scavenging capacity of GSH under thermal treatment, high salt density, and long-term storage was significantly decreased, while its Cr(VI) reduction activity was relatively stable. No significant change of Se-MPS antioxidant activity was observed under different conditions, except under citric acid. Meanwhile, its Cr(VI) reduction activity was partially or mostly retained under different treatment conditions. However, it displayed negligible ABTS+ scavenging capacity. Se-MPS was superior to GSH and MPS in terms of stability and antioxidant activity and could be a candidate for development of nutraceuticals or functional food. The resource utilization of soybean seed coats is currently poor. In this study, steam flash explosion (SFE) pretreatment was performed to extract valuable phytochemicals from soybean seed coats. The total content of phytochemicals and the antioxidant activity of extracts from SFE-treated soybean seed coat were systematically evaluated. On the basis of the application value of antioxidant activity, we optimized the process parameters of SFE-pretreated soybean seed coat to maximize the antioxidant activity. Additionally, the subsequently obtained ethyl acetate fraction with the highest antioxidant activity was analysed using HPLC-DAD-Q-Orbitrap HRMS/MS analysis. The results indicated that SFE could enhance the release of both aglycone and acetylglucoside forms of isoflavones from the cellular structure and enhance the antioxidant activity of soybean seed coats. This study provides evidence that SFE is a novel thermal processing technology with high efficiency and low energy consumption that improves the phytochemical composition and bioactivity of soybean seed coats. Native Lotus seed (NLS) starch was independently subjected to two different modifications such as heat-moisture treatment (HMT) and citric acid treatment (CAT). The effect of the treatment on physical, chemical, morphological, thermal, pasting and gelling properties of native and modified starches were evaluated during the study. The results showed that the enthalpies of the HMT and the CAT samples along with the onset, peak, and conclusion temperatures of gelatinization were increased. The FTIR analysis revealed that HMT and CAT increased the degree of order and the degree of the double helix of the NLS. The gel elasticity and the adhesiveness of the HMT and the CAT starches were also greater than the NLS starch samples. The developed modified starches could be used for enhancement of different functional properties for applying as gelling, thickening, stabilizing and filling agents for developing starch-based food formulations.