https://www.selleckchem.com/products/lyn-1604.html 01), but an increased p-Akt/Akt ratio (P less then 0.05) and an elevated level of p-mTOR phosphorylation (P less then 0.05). CONCLUSIONS Triptolide can decrease the activity and induce the apoptosis of TM3 Leydig cells, and meanwhile inhibit the AMPK and activate the Akt/mTOR pathway.Objective To identify differentially expressed proteins in the sperm of oligoasthenozoospermia (OAZ) patients and provide some theoretical evidence for the study of OAZ. METHODS We collected semen samples from 30 OAZ patients and another 30 normal healthy males. Using the tandem mass tag (TMT) and proteomic technology, we identified differentially expressed proteins in the sperm of the OAZ patients and normal subjects, followed by gene ontology (GO) analysis and bioinformatics analysis of the Kyoto encyclopedia of genes and genomes (KEGG) signaling pathways. RESULTS A total of 1 199 differentially expressed sperm proteins were obtained from the semen samples of the subjects by proteomic technology, of which 663 were up-regulated and 536 down-regulated. GO analysis preliminarily indicated that the differential proteins played a leading role in the composition and function of the ribosome, while KEGG pathway analysis showed that the differential proteins were involved in 244 signaling pathways. CONCLUSIONS Differentially expressed proteins in the sperm of OAZ patients involve complex biological processes, molecular functions and signaling pathways, and proteomic screening and bioinformatics analysis are helpful for the study of the pathogenesis of oligoasthenozoospermia.Objective To investigate the feasibility of constructing a mouse model of spermatogonial stem cell (SSC) transplant recipient by high-temperature heat stress. METHODS Four-week-old C57BL/6 male mice and B6(Cg)-Tyrc-2J/J coat color gene homozygous mutant male mice were heat-treated at 43 ℃ for an hour in the incubator. The best transplantation time was determined by HE stai