https://www.selleckchem.com/products/gsk3685032.html The null model revealed that homogeneous and variable selection were the dominant assembly processes for rare subcommunities compared with abundant species. pH and nitrogen content were the paramount drivers determining the assembly of microbial communities and functional genes, consistent with the importance of environmental filtering. Furthermore, the rare biosphere had a paramount role in the entire ecological network and was the major driver for most soil processes such as C, N, and S cycling. Nonetheless, a significant portion of soil P cycling was regulated by abundant taxa. Collectively, our study provides insight into the mechanisms underlying microbial community assembly and soil microbe-driven functional changes in biogeochemical processes during secondary succession.We examined site patterns in bee species for diversity and functional diversity in urban, suburban and rural areas. We sampled bees from all three habitat types and compiled a database of functional traits for each species. While species diversity decreased with urbanization, as expected, components of functional diversity showed differences between urban and suburban habitats. Functional dispersion (FDis) increased significantly in suburban areas as compared to urban sites, while functional divergence (FDiv) and functional redundancy (Fred) were higher in urban areas. Functional richness (FRic) and evenness (FEven) were not affected by urbanization. Moreover, assemblages in highly urbanized environments have a substantially different functional composition. Solitary species, cleptoparasites, soil nesters, bees with trophic specialization, and those with a short flight period turned out to be more sensitive to urbanization changes. This study highlights the importance of examining functional diversity in assessing human-induced biodiversity loss and its impacts on ecosystem functioning in urbanized areas. These results have significant impli