d psychological burden during the COVID-19 pandemic, having higher levels of stress, anxiety, and depression than second-line medical personnel. Adequate measures should be taken to relieve this burden and preserve the mental health of frontline medical personnel.This study evaluated the gene expression profile of the human adipose-derived stem cells (hASCs) grown on the Biosilicate® /F18 glass (BioS-2P/F18) scaffolds. hASCs were cultured using the osteogenic medium (control), the scaffolds, and their ionic extract. We observed that ALP activity was higher in hASCs grown on the BioS-2P/F18 scaffolds than in hASCs cultured with the ionic extract or the osteogenic medium on day 14. Moreover, the dissolution product group and the control exhibited deposited calcium, which peaked on day 21. Gene expression profiles of cell cultured using the BioS-2P/F18 scaffolds and their extract were evaluated in vitro using the RT2 Profiler polymerase chain reaction (PCR) microarray on day 21. Mineralizing tissue-associated proteins, differentiation factors, and extracellular matrix enzyme expressions were measured using quantitative PCR. The gene expression of different proteins involved in osteoblast differentiation was significantly up-regulated in hASCs grown on the scaffolds, especially BMP1, BMP2, SPP1, BMPR1B, ITGA1, ITGA2, ITGB1, SMAD1, and SMAD2, showing that both the composition and topographic features of the biomaterial could stimulate osteogenesis. This study demonstrated that gene expression of hASCs grown on the scaffold surface showed significantly increased gene expression related to hASCs cultured with the ionic extract or the osteogenic medium, evidencing that the BioS-2P/F18 scaffolds have a substantial effect on cellular behavior of hASCs.
  The prothrombin time (PT) is the most requested test to investigate patients with congenital or acquired coagulopathies or to monitor oral anticoagulant therapy. However, thromboplastins can show markedly different responsiveness to the defects induced by vitamin K antagonist (VKA) therapy and are thus characterized by their ISI (International Sensitivity Index). INR results are optimal for patients under VKA but for patients screened for other reasons expressing PT results as ratio can be more appropriate. As it is very difficult to define the PT results reporting unit from the PT testing request, it would be ideal to use a thromboplastin with ISI=1. The study aims to compare our reference PT reagent with two candidate thromboplastins with ISI close to 1.
 
  We compared 3 different thromplastins two rabbit brain extracted based reagents (STA-Neoplastine CI Plus, with ISI=1.26, routinely used in our laboratory and STA-NeoPTimal with ISI=1.01) and a recombinant thromboplastin (STA-Neoplastine R with ISI=0.97). The comparison was done on 175 samples 75 from individuals without coagulation defects and 100 from patients under VKA.
 
  STA-NeoPTimal and STA-Neoplastine R well correlate to our reference, STA-Neoplastine CI Plus regression equations are y=1.186x-0.1351, r
  =.9454 and y=1.1432x-0.1554, r
  =.9951, respectively. The lowest bias on INR results was obtained with STA-NeoPTimal reagent (interval -0.7/+0.4).
 
  We conclude that STA-NeoPTimal can be used in the laboratory as it gives results comparable to those obtained with STA-Neoplastine CI Plus. Besides, thanks to its ISI=1, it guarantees reporting a PT ratio equal to INR which avoids errors.
 We conclude that STA-NeoPTimal can be used in the laboratory as it gives results comparable to those obtained with STA-Neoplastine CI Plus. Besides, thanks to its ISI = 1, it guarantees reporting a PT ratio equal to INR which avoids errors.
  This study evaluates the association between BMI and all-cause and cause-specific mortality in South Africa.
 
  Prospective, population-based observational cohort data from rural South Africa were analyzed. BMI was measured in 2010. Demographic characteristics were recorded and deaths were verified with verbal autopsy interview. The InterVA-5 tool was used to assign causes of death. HIV testing was conducted annually. Cox proportional hazards models were fit to estimate the effect of BMI on all-cause and cause-specific mortality, accounting for the competing risk of death from other causes. Models were adjusted for sociodemographic characteristics and HIV status, and inverse probability weighting for survey nonparticipation was used.
 
  The cohort consisted of 9,728 individuals. In adjusted models, those with BMI of 25.0 to 29.9 kg/m
  or 30.0 to 34.9 kg/m
  had a lower hazard of death (adjusted hazard ratio 0.80; 95% CI 0.69-0.92 and adjusted hazard ratio 0.75; 95% CI 0.60-0.93, respectively) compared with those with BMI of 18.5 to 24.9 kg/m
  .
 
  Individuals in South Africa who meet clinically defined criteria for overweight or obesity had a lower risk of all-cause mortality than those with a normal BMI. These findings were stronger for women and communicable conditions.
 Individuals in South Africa who meet clinically defined criteria for overweight or obesity had a lower risk of all-cause mortality than those with a normal BMI. These findings were stronger for women and communicable conditions.
  Huntington's disease (HD) is a heritable degenerative brain disease caused by a mutation in the huntingtin gene with excessive repeats of the base triplet cytosine-adenine-guanine (CAG), which codes for the aminoacid glutamine.  https://www.selleckchem.com/products/heparan-sulfate.html  HD is associated with a broad spectrum of neurocognitive dysfunction, including deficits in social cognition. The appreciation of fairness rules and reciprocity has not been studied in HD. Based on theoretical considerations suggesting that brain regions known to be affected from HD are involved in economic decision-making, the present study sought to examine HD patients' performance in two neuroeconomic games.
 
  Twenty-nine manifest HD mutation carriers (20 males, nine females) performed an Ultimatum Game (UG) and a Dictator Game (DG) where third-party punishment of observed unfairness was required. In addition, patients were tested for neurocognition and the ability to understand other people's mental states ("theory of mind"). For comparison, a clinical control group of 30 patients with chronic schizophrenia, and 30 unaffected healthy controls matched for age and verbal intelligence took part in the study.